Detection of inapparent nodule-transformed cells in the mammary gland tissues of virgin female BALB/cfC3H mice.
Detection of inapparent nodule-transformed cells in the mammary gland tissues of virgin female BALB/cfC3H mice.
复制标题
检测处女雌性 BALB/cfC3H 小鼠乳腺组织中不明显的结节转化细胞。
作者：
K. Deome;M. Miyamoto;R. Osborn;R. Guzman;K. Lum
通过细胞解离技术，在增生性肺泡结节（HAN）或肿瘤出现之前，从未交配雌性BALB/cfC 3 H/Crgl（小鼠乳腺肿瘤阳性）小鼠的形态正常乳腺组织中回收不明显的结节转化细胞。通过胶原酶（0.1%）、透明质酸酶（0.1%）和链霉蛋白酶（1.25%）分离乳腺组织。将0.01 ml培养基中的10（5）个活细胞等分试样注射到3周龄雌性同系小鼠的无腺体乳腺脂肪垫中。10周后，检查生长物并将其分类为导管、HAN、肿瘤或这些类型的组合。HAN副产物的存在表明供体乳腺组织中存在结节转化细胞。从2个月大的供体中回收结节转化细胞，并且HAN副产物的数量随着供体年龄的增加而增加。明显的HAN和肿瘤未出现在年龄小于8至9个月的处女雌性BALB/cfC 3 H/Crgl小鼠中。这些数据表明，不明显的结节转化细胞早在肿瘤HAN出现之前就已出现，并且转化细胞的数量随供体年龄的增加而增加。
Inapparent nodule-transformed cells were recovered from morphologically normal mammary tissue from virgin female BALB/cfC3H/Crgl (mouse mammary tumor-positive) mice before the appearance of hyperplastic alveolar nodules (HAN) or tumors, by means of the cell dissociation technique. Mammary tissues were dissociated by means of collagenase (0.1%), hyaluronidase (0.1%), and pronase (1.25%). Aliquots of 10(5) viable cells in 0.01 ml medium were injected into the gland-free mammary fat pads of 3-week-old female syngeneic mice. After 10 weeks the outgrowths were examined and classified as ductal, HAN, tumor, or combinations of these types. The presence of HAN outgrowths indicated the presence of nodule-transformed cells in the donor mammary tissues. Nodule-transformed cells were recovered from 2-month-old donors, and the number of HAN outgrowths increased with donor age. Overt HAN and tumors did not appear in virgin female BALB/cfC3H/Crgl mice younger than 8 to 9 months of age. The data suggest that inapparent nodule-transformed cells occurred long before the appearance of HAN of tumors and that the numbers of transformed cells increased with donor age.