Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression
Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression
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RpoS 在假结核耶尔森菌应激存活、运动、生物膜形成和 VI 型分泌系统表达中的作用
DOI：
10.1007/s12275-015-0099-6
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发表时间：
2015-09-01
影响因子：
3
通讯作者：
Wang, Yao
中科院分区：
文献类型：
--
作者：
Guan, Jingyuan;Xiao, Xiao;Wang, Yao
RpoS（sigma（S）），稳定期/应激sigma因子，控制参与对各种应激的细胞应答的大量基因的表达。然而，RpoS的作用似乎在不同的细菌中有所不同。虽然RpoS是鞭毛生物合成的重要调节因子，但它与迟缓爱德华氏菌中的生物膜发育相关。生物膜是由细菌形成的密集群落，对微生物在不利条件下的生存至关重要。据报道，最近发现的VI型分泌系统（T6 SS）与几种表型相关，从生物膜形成到压力感应。例如，鳗弧菌T6 SS被提出作为细胞质外信号的传感器，并调节RpoS表达和应激反应。在这项研究中，我们研究了RpoS在假结核耶尔森氏菌中的生理作用，包括压力条件下的细菌存活，鞭毛形成，生物膜发育和T6 SS表达。我们发现RpoS在Y.假结核此外，我们的研究表明，RpoS不仅调控T6 SS的表达，还通过正向调控鞭毛主调控基因flhDC来调控鞭毛的形成，并通过调控胞外多糖的合成来影响秀丽隐杆线虫生物膜的形成。总之，这些结果表明，RpoS在Y中的几种不利条件下在细胞适应性中起着核心作用。假结核
RpoS (sigma(S)), the stationary phase/stress sigma factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. However, the role of RpoS appears to differ in different bacteria. While RpoS is an important regulator of flagellum biosynthesis, it is associated with biofilm development in Edwardsiella tarda. Biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. The type VI secretion system (T6SS) discovered recently is reportedly associated with several phenotypes, ranging from biofilm formation to stress sensing. For example, Vibrio anguillarum T6SS was proposed to serve as a sensor for extracytoplasmic signals and modulates RpoS expression and stress response. In this study, we investigated the physiological roles of RpoS in Yersinia pseudotuberculosis, including bacterial survival under stress conditions, flagella formation, biofilm development and T6SS expression. We found that RpoS is important in resistance to multiple stressors-including H2O2, acid, osmotic and heat shock-in Y. pseudotuberculosis. In addition, our study showed that RpoS not only modulates the expression of T6SS but also regulates flagellum formation by positively controlling the flagellar master regulatory gene flhDC, and affects the formation of biofilm on Caenorhabditis elegans by regulating the synthesis of exopolysaccharides. Taken together, these results show that RpoS plays a central role in cell fitness under several adverse conditions in Y. pseudotuberculosis.