Pea Ty1-copia group retrotransposons:: transpositional activity and use as markers to study genetic diversity in Pisum
Pea Ty1-copia group retrotransposons:: transpositional activity and use as markers to study genetic diversity in Pisum
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DOI：
10.1007/s004380000257
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发表时间：
2000-07-01
期刊：
影响因子：
--
通讯作者：
Kumar, A
中科院分区：
文献类型：
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作者：
Pearce, SR;Knox, M;Kumar, A
研究了不同Ty 1-copia组LTR反转录转座子在豌豆属物种谱系中转座历史的变化。通过简并PCR分离出Ty 1-copia元件的异质群体，并根据其拷贝数和密切相关物种之间的序列保守性选择其中的两个（Tps 12和Tps 19），以进一步深入研究其在豌豆属物种中的转座历史。序列特异性扩增多态性（SSAP）研究了这些元素和先前表征的PDR 1元素的插入多态性。这些元素中的每一个揭示了55个不同的豌豆加入一个独特的易位历史。基于SSAP数据的系统发育树表明，SSAP标记的个别元素能够解决不同的物种谱系内豌豆属。最后，结合所有这些反转录转座子标记的SSAP数据，揭示了豌豆种内和种间关系的详细情况。
The variation in transposition history of different Ty1-copia group LTR retrotransposons in the species lineages of the Pisum genus has been investigated. A heterogeneous population of Ty1-copia elements was isolated by degenerate PCR and two of these (Tps12 and Tps19) were selected on the basis of their copy number and sequence conservation between closely related species for further in-depth study of their transpositional history in Pisum species. The insertional polymorphism of these elements and the previously characterised PDR1 element was studied by sequence-specific amplification polymorphism (SSAP). Each of these elements reveals a unique transpositional history within 55 diverse Pisum accessions. Phylogenetic trees based on the SSAP data show that SSAP markers for individual elements are able to resolve different species lineages within the Pisum genus. Finally, the SSAP data from all of these retrotransposon markers were combined to reveal a detailed picture of the intra and interspecies relationships within Pisum.