Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.
Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.
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使用 DEAE 纸从琼脂糖凝胶中分离 DNA。
DOI：
10.1093/nar/8.2.253
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发表时间：
1980
影响因子：
14.9
通讯作者：
M. Hammarskjöld
中科院分区：
文献类型：
--
作者：
G. Winberg;M. Hammarskjöld
介绍了一种从琼脂糖凝胶中分离DNA的新方法。该方法包括将琼脂糖平板凝胶中的所有dna片段同时转移到deae -纤维素纸上，并用1m NaCl从纸上洗脱单个片段。用这种方法从琼脂糖凝胶中分离的DNA容易被几种限制性内切酶切割，并且可以用大肠杆菌DNA-聚合酶I、T4 DNA-聚合酶和T4多核苷酸激酶进行体外标记。我们用这种方法构建了腺病毒16型DNA的限制性内切酶图谱。
A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of the individual fragments from the paper with 1 M NaCl. DNA isolated from agarose gels in this way is susceptible to cleavage with several restriction endonucleases, and can be labeled in vitro with E coli DNA-polymerase I, T4 DNA-polymerase and T4 polynucleotide kinase. We have used the method to construct restriction endonuclease maps of adenovirus type 16 DNA.