Desmoplakin is essential in epidermal sheet formation
Desmoplakin is essential in epidermal sheet formation
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DOI：
10.1038/ncb1201-1076
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发表时间：
2001-12-01
影响因子：
21.3
通讯作者：
Fuchs, E
中科院分区：
文献类型：
--
作者：
Vasioukhin, V;Bowers, E;Fuchs, E
我们已经产生了一个表皮特异性桥粒斑蛋白（DP）敲除小鼠，并表明表皮完整性需要DP;机械应力DP-空皮肤引起细胞间分离。无DP皮肤中表皮桥粒的数量与野生型（WT）相似，但它们缺乏角蛋白丝，这损害了它们的功能。无DP的角质形成细胞在体外几乎没有桥粒，并且在上皮片形成期间不能进行肌动蛋白重组和膜封闭。粘附连接也减少。在体外，DP转基因表达挽救了这些缺陷。因此，DP是组装功能性桥粒所必需的，维持细胞骨架结构并加强稳定细胞间粘附所必需的膜附着。
We have generated an epidermis-specific desmoplakin (DP) mouse knockout, and show that epidermal integrity requires DP; mechanical stresses to DP-null skin cause intercellular separations. The number of epidermal desmosomes in DP-null skin is similar to wild type (WT), but they lack keratin filaments, which compromise their function. DP-null keratinocytes have few desmosomes in vitro, and are unable to undergo actin reorganization and membrane sealing during epithelial sheet formation. Adherens junctions are also reduced. In vitro, DP transgene expression rescues these defects. DP is therefore required for assembly of functional desmosomes, maintaining cytoskeletal architecture and reinforcing membrane attachments essential for stable intercellular adhesion.