Identifying Pig Mitochondrial TSS: Structure and Functional Features
Identifying Pig Mitochondrial TSS: Structure and Functional Features
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鉴定猪线粒体 TSS：结构和功能特征
DOI：
10.1016/j.mito.2019.07.001
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发表时间：
2019-11-01
期刊：
影响因子：
4.4
通讯作者：
Zhao, Xingbo
中科院分区：
文献类型：
--
作者：
Liu, Hao;Yin, Tao;Zhao, Xingbo
本研究首次鉴定了猪线粒体基因组转录起始位点（TSS），包括位于H链第903位和第1369位的重链启动子1和2（HSP 1和HSP 2），以及位于L链第166位的轻链启动子（LSP）。通过分析线粒体DNA拷贝数、11个核质基因的表达、线粒体DNA甲基化水平以及甲基化修饰酶DNMT 1和THBG的基因表达水平，研究了HSP 1的结构和表达特征。线粒体DNA拷贝数在15种器官/组织中存在较大差异，其中胰腺（与1890相对拷贝数相近）和脾脏（与110相对拷贝数相近，P
The transcription start sites (TSSs) of porcine mitochondrial genome were firstly identified in this study, including heavy-strand promoter 1 and 2 (HSP1 and HSP2) harbored at nt 903 and nt 1369 in H strand, respectively, and light-strand promoter (LSP) located at nt 166 in L strand. HSP1 structure and expression features were investigated by analyzing mtDNA copy number, expression of 11 nucleoplasmic genes, mtDNA methylation levels, and gene expression levels of methyl-modifying enzymes, DNMT1 and TETs. The mtDNA copy number presented large differences among 15 organs/tissues, and the largest disparity, nearly 17 times, was found between pancreas (similar to 1890 relative copy numbers) and spleen (similar to 110 relative copy numbers, P