Relationship of structure to function in bacterial endotoxins: serologically cross-reactive components and their effect on protection of mice against some gram-negative infections.
Relationship of structure to function in bacterial endotoxins: serologically cross-reactive components and their effect on protection of mice against some gram-negative infections.
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细菌内毒素结构与功能的关系：血清学交叉反应成分及其对保护小鼠免受某些革兰氏阴性菌感染的影响。
DOI：
10.1099/00221287-94-1-107
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
A. Nowotny
中科院分区：
文献类型：
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作者：
A. Ng;C. L. Chen;C. Chang;A. Nowotny
制备了抗无肝Re突变体沙门氏菌R595和沙门氏菌S.鼠伤寒沙门氏菌SLI102，以及对纯化的R595糖脂包被自体红细胞。抗血清与从各种细菌菌株的Re突变体中提取的内毒素糖脂发生交叉反应，包括S。miniorR595、S.鼠伤寒沙门氏菌SLI102、大肠杆菌D3Im4、E. coli D2If2和E.大肠杆菌F515，通过被动血凝试验和凝胶扩散试验证明。抗Re血清还与RESI制剂（纯化的“脂质A”级分）发生交叉反应，RESI制剂来自各种异源光滑革兰氏阴性细菌（包括粘质沙雷氏菌）的内毒素脂多糖。荧光假单胞菌和E. coli 0127。然而，相同的抗血清不能保护小鼠免受革兰氏阴性菌如肺炎克雷伯氏菌II型、S.伤寒0901、铜绿假单胞菌119和大肠杆菌。杆菌结果表明，虽然革兰氏阴性菌的细胞壁中的脂多糖的脂质部分共享交叉反应的免疫决定簇基团，这些基团可能无法访问针对它们的抗体。
Rabbit antisera were prepared against the heptoseless Re mutants, Salmonella minnesota R595 and S. typhimurium SLI102, as well as against purified R595 glycolipid coated on autologous erythrocytes. The antisera cross-reacted with the endotoxic glycolipids extracted from Re mutants of various bacterial strains, including S. minnesota R595, S. typhimurium SLI102, Escherichia coli D3Im4, E. coli D2If2 and E. coli F515, as shown by passive haemagglutination and gel diffusion tests. The anti-Re sera also cross-reacted with the RESI preparations (a purified 'lipid A' fraction) from the endotoxic lipopolysaccharides of various heterologous smooth Gram-negative bacteria including Serratia marcescens. Psuedomonas fluorescens and E. coli 0127. However, the same antisera failed to protect mice against infection by Gram-negative bacteria such as Klebsiella pneumoniae type II, S. typhi 0901, P. aeruginosa 119 and E. coli. The results suggest that although the lipid moieties of the lipopolysaccharides in the cell wall of Gram-negative bacteria share cross-reactive immunodeterminant groups, these groups may not be accessible to antibody against them.