Analysis of the Monosaccharide Composition of Purified Polysaccharides in Ganoderma atrum by Capillary Gas Chromatography
Analysis of the Monosaccharide Composition of Purified Polysaccharides in Ganoderma atrum by Capillary Gas Chromatography
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DOI：
10.1002/pca.1153
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发表时间：
2009-11-01
影响因子：
3.3
通讯作者：
Li, Chang
中科院分区：
文献类型：
--
作者：
Chen, Yi;Xie, Ming-Yong;Li, Chang
灵芝是我国最著名的传统中药之一，从其中分离出的数十种多糖显示出多种多样的潜在药理活性，引起了人们的广泛关注。然而，对灵芝水提物中多糖单糖组成的分析尚未见报道。目的建立一种简便、灵敏的气相色谱法分析灵芝纯化多糖中单糖组成的方法。方法-首先将多糖水解得到单糖成分，随后将其衍生为乙酰化的醛腈，并使用毛细管柱填充（5%苯基）甲基聚硅氧烷固定相，并添加乙酰肌醇作为内标，进行气相色谱分析。采用高效液相色谱法进行比较。结果-大多数常见单糖的稳定衍生物可以被分离，并且具有高灵敏度和重复性。检测限为0.013 mg/mL，定量限为0.043 mg/mL。中间精度值（以RSD表示）小于10%。该方法平均回收率为100±3%，RSD值小于5%。结果表明，气相色谱法和高效液相色谱法的测定结果在可接受的误差范围内接近。结论：该方法可作为一种准确的单糖组成分析方法，应用于生物和生化研究领域。版权所有John Wiley & Sons， Ltd. 2009
Introduction - Ganoderma, one of the best-known traditional Chinese medicines, has attracted considerable attention owing to the fact that dozens of polysaccharides isolated from it have shown diverse and potentially significant pharmacological activities. However, no work has been reported on the analysis of monosaccharide composition of polysaccharide isolated from the aqueous extract of Ganoderma atrum yet.Objective - To develop a simple and sensitive GC-based method for the analysis of monosaccharide composition of purified polysaccharides in Ganoderma atrum.Methodology - The polysaccharide was first hydrolysed to give the constituent monosaccharides, which were subsequently derived into acetylated aldononitriles and analysed by gas chromatography using a capillary column packed with a (5%phenyl) methylpolysiloxane stationary phase with the addition of acetyl inositol as the inner standard. High-performance liquid chromatography was also used for comparison.Results - The stable derivatives of the most common monosaccharides could be separated and reproducibly determined with high sensitivity. The limits of detection and quantification were 0.013 and 0.043 mg/mL, respectively. The intermediary precision values (expressed as the RSD) were less than 10%. The mean recovery of the method was 100 +/- 3%, with RSD values of less than 5%. The results obtained from GC and HPLC methods were found to be close to each other within acceptable error ranges.Conclusion - This study demonstrated that the developed method could be applied as an accurate method for the compositional analysis of monosaccharides in the field of biological and biochemical study. Copyright (C) 2009 John Wiley & Sons, Ltd.