Skeletal muscle fibre type transformation following spinal cord injury
Skeletal muscle fibre type transformation following spinal cord injury
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DOI：
10.1038/sj.sc.3100364
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发表时间：
1997-02-01
期刊：
影响因子：
2.2
通讯作者：
Steadward, R
中科院分区：
文献类型：
--
作者：
Burnham, R;Martin, T;Steadward, R
脊髓损伤（SCI）后，上运动神经元麻痹的肌肉失去了正常的I型（慢）和II型（快）纤维镶嵌模式，并主要由II型（快速糖酵解）纤维组成。大多数证明这种纤维类型转变的研究是基于长期截瘫和四肢瘫痪患者肌肉的pH敏感性肌原纤维ATP酶染色技术。本研究的目的是描述肌纤维类型的变化在一个广泛的时间范围后SCI使用免疫荧光技术，可能是更敏感的变化。从12名SCI受试者中总共获得了19份股外侧肌活检标本，代表SCI后0.5-219个月的时间点。快肌球蛋白重链和慢肌球蛋白重链异构体分布测定单个肌纤维的活检标本。2015年10月20日，美国国家航空航天局（
Following spinal cord injury (SCI), upper motor neuron paralysed muscles lose the normal type I (slow) and II (fast) fibre mosaic pattern and become predominantly composed of type II (fast glycolytic) fibres). The majority of the research demonstrating this fibre type shift was based on pH sensitive myofibrillar ATPase staining techniques on muscle from longstanding paraplegics and quadriplegics. The purpose of this study was to describe muscle fibre type changes over a wide time spectrum post SCI using immunofluorescent techniques which may be more sensitive to change. A total of 19 vastus lateralis muscle biopsy specimens were obtained from 12 SCI subjects representing time points of 0.5-219 months post SCI. Fast and slow myosin heavy chain isoform distribution was determined on single muscle fibres for each of the biopsy specimens. Early post SCI (