TRANSLATIONAL REGULATION AND DEADENYLATION OF A PROTAMINE MESSENGER-RNA DURING SPERMIOGENESIS IN THE MOUSE
TRANSLATIONAL REGULATION AND DEADENYLATION OF A PROTAMINE MESSENGER-RNA DURING SPERMIOGENESIS IN THE MOUSE
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DOI：
10.1016/0012-1606(84)90262-8
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
HECHT, NB
中科院分区：
文献类型：
--
作者：
KLEENE, KC;DISTEL, RJ;HECHT, NB
用北方印迹法检测了2种小鼠精蛋白中的1种，即富含半胱氨酸、含酪氨酸的精蛋白（MP 1）在总睾丸和纯化的圆形和伸长精子细胞群体的多聚体和非多聚体区室中的mRNA分布。在从总睾丸制备的线粒体后上清液中，10-15%的MP 1-mRNA沉积在小的多聚核糖体上。MP 1-mRNA的非多聚体分子大小均一，580个碱基，而多聚体分子是异质的，具有apprx模式。450个基地用RNA酶H消化和在聚（U）琼脂糖上的热色谱法揭示了多聚体和非多聚体MP 1-mRNA大小的差异是由于聚（A）从λ缩短所致。160到30个碱基在圆形精子细胞中，基本上所有的MP 1-mRNA是580个碱基长，是在非多聚体部分。伸长精子细胞含有大致相等比例的均匀，580个碱基的形式在非多聚体室，和异质450个碱基的形式只在多聚体室。显然，小鼠鱼精蛋白中的一种的mRNA在圆形精子细胞中以未翻译的RNP形式储存，并且当其在伸长精子细胞中翻译时部分去腺苷化。
The distribution of the mRNA for 1 of the 2 mouse protamines, the cysteine-rich, tyrosine-containing protamine (MP1), was examined in the polysomal and nonpolysomal compartments of total testis and purified populations of round and elongating spermatids using Northern blots. In postmitochondrial supernatants prepared from total testis, .apprx. 10-15% of MP1-mRNA sediments with the small polysomes. The nonpolysomal molecules of MP1-mRNA are homogeneous in size, .apprx. 580 bases, while the polysomal molecules are heterogeneous with a mode of .apprx. 450 bases. Digestion with RNase H and thermal chromatography on poly (U) Sepharose reveals that the difference in size of polysomal and nonpolysomal MP1-mRNA is due to a shortening of the poly(A) from .apprx. 160 to 30 bases. In round spermatids, essentially all of MP1-mRNA is 580 bases long and is in the nonpolysomal fraction. Elongating spermatids contain roughly equal proportions of the homogeneous, 580 base form in the nonpolysomal compartment, and the heterogeneous 450 base form solely in the polysomal compartment. Apparently mRNA for 1 of the mouse protamines is stored as an untranslated RNP in round spermatids, and it is partially deadenylated when it is translated in elongating spermatids.