Influence of Body Condition on Serum Metabolic Indicators of Lipid Mobilization and Oxidative Stress in Dairy Cows During the Transition Period
Influence of Body Condition on Serum Metabolic Indicators of Lipid Mobilization and Oxidative Stress in Dairy Cows During the Transition Period
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DOI：
10.1111/rda.12608
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发表时间：
2015-12-01
影响因子：
1.7
通讯作者：
Samardzija, M.
中科院分区：
文献类型：
--
作者：
Folnozic, I.;Turk, R.;Samardzija, M.
本研究的目的是研究奶牛的身体状况对代谢和抗氧化状态的影响，以及过渡期脂肪动员代谢指标与氧化应激的关系。本研究选用24头荷斯坦-弗里斯奶牛，根据其体况评分(BCS)分为两组：最佳体况评分组(n=12；BCS=3.25~3.75)和肥胖组(n=12；BCS&Gt；=4)。分别于分娩后30天、10天、2天、0天、5天、12天、19天、26天和60天采血分析代谢状态(葡萄糖、甘油三酯、总胆固醇、高密度脂蛋白、NEFA和BHb)、对氧磷酶-1(PON1)和载脂蛋白A-I(ApoA-I)。产后12天和19天，脂肪奶牛的血糖水平显著高于对照组，产后12天和19天显著降低。两组奶牛的总胆固醇和高密度脂蛋白胆固醇水平在分娩时最低，在产后26天和60天显著高于对照组。从分娩到分娩后第19天，两组受试者的NEFA浓度都显著升高，这表明产后能量不足和脂质动员增加。两组过渡期BHb浓度差异无统计学意义。两组奶牛在过渡期的PON1活性和ApoA-I浓度均无显著差异。然而，在肥胖奶牛中，尽管没有显著差异，但PON1从产后到分娩后第19天都有所下降，这表明肥胖奶牛的抗氧化状态受到了干扰。PON1与总胆固醇、高密度脂蛋白胆固醇浓度呈显着正相关，与NEFA呈显着负相关，提示PON1与脂质代谢密切相关。两组奶牛的NEFA和BHB呈显着正相关，表明过渡期奶牛往往通过脂肪动员来克服能量不足，为分娩和哺乳提供替代能量来源。
The objectives of this study were to examine the influence of body condition of cows on metabolic and antioxidative status, as well as to investigate the relationship between metabolic indicators of lipid mobilization and oxidative stress during transition period. The study was conducted on 24 Holstein-Friesian dairy cows divided into 2 groups according to their body condition score (BCS) as optimal (n = 12; BCS from 3.25 to 3.75) or adipose (n = 12; BCS >= 4). Metabolic status (glucose, triglycerides, total cholesterol, HDL cholesterol, NEFA and BHB), paraoxonase-1 (PON1) and apolipoprotein A-I (ApoA-I) were analysed in sera taken on days -30, -10, -2, 0, 5, 12, 19, 26 and 60 relative to parturition. Adipose cows had significantly higher glucose concentration at parturition being significantly decreased after parturition on days 12 and 19. Total cholesterol and HDL-C concentrations were the lowest at parturition and significantly higher on days 26 and 60 after parturition in both groups of cows. Both investigated groups had significantly higher NEFA concentration from parturition until day 19 after parturition, indicating energy deficit and an increased lipid mobilization after calving. There were no significant differences in BHB concentration during transition period in both groups. No significant differences were found in PON1 activity and ApoA-I concentration during transition period in both groups of cows. However, in adipose cows, although not significantly different, PON1 was decreased from calving until day 19 after parturition indicating a disturbance in antioxidative status in adipose cows. PON1 significantly positively correlated with total cholesterol and HDL-C concentrations and negatively with NEFA indicating a strong relationship of PON1 with lipid metabolism. Significant positive correlation between NEFA and BHB in both groups of cows points out on energy deficit during transition period that cows tend to overcome by lipid mobilization providing alternative source of energy needed for parturition and lactation.