Global change in brain state during spontaneous and forced walk in Drosophila is composed of combined activity patterns of different neuron classes.
Global change in brain state during spontaneous and forced walk in Drosophila is composed of combined activity patterns of different neuron classes.
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DOI：
10.7554/elife.85202
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发表时间：
2023-04-17
期刊：
影响因子：
7.7
通讯作者：
Grunwald Kadow IC
中科院分区：
文献类型：
--
作者：
Aimon S;Cheng KY;Gjorgjieva J;Grunwald Kadow IC
运动相关的大脑活动已经在不同的物种和大脑区域被发现。在这里，我们使用快速全脑光场成像在成年果蝇研究行走与全脑神经元活动之间的关系。我们观察到活动的整体变化与自发散步密切相关。虽然成像特定的兴奋性、抑制性和神经调节神经元组突出了它们的联合作用，但行走和转身诱导活动的空间异质性允许分析亚区和有时单个候选神经元的独特反应。例如，先前未表征的血清素能神经元在行走过程中受到抑制。在自发行走的动物中，一些区域的活动发生在行走开始之前，而自发行走和强迫行走在大多数大脑区域引发了类似的活动。这些数据表明，行走和行走相关的感觉或本体感受信息对所有主要神经元类的整体活动有重要贡献。
Movement-correlated brain activity has been found across species and brain regions. Here, we used fast whole brain lightfield imaging in adult Drosophila to investigate the relationship between walk and brain-wide neuronal activity. We observed a global change in activity that tightly correlated with spontaneous bouts of walk. While imaging specific sets of excitatory, inhibitory, and neuromodulatory neurons highlighted their joint contribution, spatial heterogeneity in walk- and turning-induced activity allowed parsing unique responses from subregions and sometimes individual candidate neurons. For example, previously uncharacterized serotonergic neurons were inhibited during walk. While activity onset in some areas preceded walk onset exclusively in spontaneously walking animals, spontaneous and forced walk elicited similar activity in most brain regions. These data suggest a major contribution of walk and walk-related sensory or proprioceptive information to global activity of all major neuronal classes.