Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection
Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection
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DOI：
10.1016/j.jviromet.2014.03.028
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发表时间：
2014-08-01
影响因子：
3.1
通讯作者：
Kageyama, Tsutomu
中科院分区：
文献类型：
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作者：
Nakauchi, Mina;Takayama, Ikuyo;Kageyama, Tsutomu
利用逆转录环介导等温扩增（RT-LAMP）技术建立了H7N9禽流感病毒感染的基因诊断系统。RT-LAMP检测显示与季节性甲型流感病毒（H3 N2和H1 N1 pdm 09）或在人类中传播的流感B病毒或禽流感病毒A（H5 N1）无交叉反应性。RT-LAMP检测的灵敏度为42.47拷贝/反应。该方法特异性强，灵敏度高，反应在30 min内完成，是一种很有前途的禽流感病毒快速诊断方法。(c)© 2014 Elsevier B. V.保留所有权利。
A genetic diagnosis system for detecting avian influenza A (H7N9) virus infection using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology was developed. The RT-LAMP assay showed no cross-reactivity with seasonal influenza A (H3N2 and H1N1pdm09) or influenza B viruses circulating in humans or with avian influenza A (H5N1) viruses. The sensitivity of the RT-LAMP assay was 42.47 copies/reaction. Considering the high specificity and sensitivity of the assay for detecting the avian influenza A (H7N9) virus and that the reaction was completed within 30 mm, the RT-LAMP assay developed in this study is a promising rapid diagnostic tool for avian influenza A (H7N9) virus infection. (c) 2014 Elsevier B.V. All rights reserved.