Age-related dystrophin-glycoprotein complex structure and function in the rat extensor digitorum longus and soleus muscle
Age-related dystrophin-glycoprotein complex structure and function in the rat extensor digitorum longus and soleus muscle
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DOI：
10.1093/gerona/61.11.1119
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发表时间：
2006-11-01
影响因子：
5.1
通讯作者：
Blough, Eric R.
中科院分区：
文献类型：
--
作者：
Rice, Kevin M.;Preston, Deborah L.;Blough, Eric R.
这项研究检验了这样的假设：肌营养不良蛋白-糖蛋白复合物 (DGC) 的年龄相关变化可能先于肌肉形态和功能的年龄相关变化。与成年（6 个月）大鼠相比，老年（30 个月）和非常老（36 个月）Fischer 344/NNiaHSD X Brown挪威/BiNia 大鼠的趾长伸肌 (EDL) 和比目鱼肌质量下降。 EDL 中肌营养不良蛋白、β-肌营养不良聚糖和 α-肌聚糖的含量随着年龄的增长而增加，而比目鱼肌中的肌营养不良蛋白、β-肌营养不良聚糖和 α-肌聚糖的含量则随着年龄的增长而减少。肌肉中的α-肌营养不良聚糖水平随着年龄的增长而增加，并显示出糖基化改变的证据。肌肉拉伸增加后抗体浸润和抗肌营养不良蛋白存在的免疫染色表明比目鱼肌老化的特点是膜完整性降低。总之，这些数据表明衰老与 EDL 和比目鱼肌 DGC 蛋白含量和定位的改变有关。这些结果可能表明 DGC 在与年龄相关的骨骼肌重塑中发挥着作用。
This study tested the hypothesis that age-related changes in the dystrophin-glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD X Brown Norway/BiNia rats. The amount of dystrophin, beta-dystroglycan, and alpha-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. alpha-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration and dystrophin following increased muscle stretch suggested that the aging in the soleus was characterized by diminished membrane integrity. Together, these data suggest that aging is associated with alterations in EDL and soleus DGC protein content and localization. These results may implicate the DGC as playing a role in age-associated skeletal muscle remodeling.