Inhaled Microparticles Containing Clofazimine Are Efficacious in Treatment of Experimental Tuberculosis in Mice
Inhaled Microparticles Containing Clofazimine Are Efficacious in Treatment of Experimental Tuberculosis in Mice
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DOI：
10.1128/aac.01897-12
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发表时间：
2013-02-01
影响因子：
4.9
通讯作者：
Misra, Amit
中科院分区：
文献类型：
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作者：
Verma, Rahul Kumar;Germishuizen, Willem A.;Misra, Amit
用人单核细胞来源的巨噬细胞培养物和低剂量气雾剂感染的小鼠，评价了含氯法齐明的可吸入干粉微粒(CFM-DPI)和天然氯法齐明(CFM)对结核分枝杆菌的抑制作用。两种制剂的体外杀灭率均为99%，杀灭率为2.5mU/ml。在小鼠中，每周两次吸入480mUg和720mgCFM-DPI的小鼠肺内CFU数量减少了高达对数(10)2.6；口服CFM 500mgCFM实现了对数(10)0.7的减少。
Inhalable clofazimine-containing dry powder microparticles (CFM-DPI) and native clofazimine (CFM) were evaluated for activity against Mycobacterium tuberculosis in human monocyte-derived macrophage cultures and in mice infected with a low-dose aerosol. Both formulations resulted in 99% killing at 2.5 mu g/ml in vitro. In mice, 480 mu g and 720 mu g CFM-DPI inhaled twice per week over 4 weeks reduced numbers of CFU in the lung by as much as log(10) 2.6; 500 mu g oral CFM achieved a log(10) 0.7 reduction.