Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections
Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections
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DOI：
10.1111/j.1574-695x.2011.00821.x
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发表时间：
2011-10-01
影响因子：
--
通讯作者：
Komiya, Setsuro
中科院分区：
文献类型：
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作者：
Kawamura, Hideki;Nishi, Junichiro;Komiya, Setsuro
生物膜在医疗器械相关感染中起着关键作用。然而，骨科感染患者临床耐甲氧西林金黄色葡萄球菌（MRSA）生物膜形成和基因分型的流行病学分析很少有报道。共检测了168株MRSA菌株：23株来自器械相关感染患者（器械组）；非器械相关感染患者55例（非器械组）；无症状鼻带菌者（定植组）90例。采用脉冲场凝胶电泳分析和agr分型等5种基因分型方法。采用微滴板法定量测定生物膜的形成。装置组agr-2的发生率明显高于定殖组（78.3% vs. 34.4%, P = 0.001）。agr-2菌株的生物膜指数（0.523 +/- 0.572）显著高于agr-1菌株（0.260 +/- 0.418,P < 0.0001）和agr-3菌株（0.379 +/- 0.557,P = 0.045）。器械组强生物成膜率（43.5%）显著高于非器械组（12.7%,P = 0.003）和定植组（20.0%,P = 0.020）。ag -2型MRSA菌株具有较强的生物膜形成能力，更容易引起骨科器械感染。
Biofilms play a pivotal role in medical device-related infections. However, epidemiological analysis of biofilm formation and genotyping among clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients with orthopaedic infections has rarely been reported. A total of 168 MRSA strains were examined: 23 strains from patients with device-related infection (the device group); 55 from patients with device-non-related infection (the nondevice group); and 90 from asymptomatic nasal carriers (the colonization group). Pulsed-field gel electrophoresis analysis and five genotyping methods including agr typing were performed. Biofilm formation was quantified using a microtitre plate assay. The device group had a significantly higher incidence of agr-2 than the colonization group (78.3% vs. 34.4%, P = 0.001). The biofilm index of the agr-2 (0.523 +/- 0.572) strains was significantly higher than those of agr-1 (0.260 +/- 0.418, P < 0.0001) and agr-3 (0.379 +/- 0.557, P = 0.045). The prevalence of strong biofilm formers in the device group (43.5%) was significantly higher than that in the nondevice group (12.7%, P = 0.003) and the colonization group (20.0%, P = 0.020). agr-2 MRSA strains may be more likely to cause orthopaedic device infection because of their strong biofilm formation ability.