Gene ontology functional annotations at the structural domain level
Gene ontology functional annotations at the structural domain level
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DOI：
10.1002/prot.22373
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发表时间：
2009-08-15
影响因子：
2.9
通讯作者：
Pazos, Florencio
中科院分区：
文献类型：
--
作者：
Lopez, Daniel;Pazos, Florencio
大多数蛋白质被组织在结构域中，这些结构域可以被视为分子功能（MF）方面的独立模块单元。然而，目前的功能注释是在“全链”的基础上进行的，而没有将特定功能与各个结构域相关联。我们在这里提出了一个自动识别的方法，在一个蛋白质内的特定结构域是负责一个给定的MF最初归因于整个蛋白质。通过使用该方法对SCOP结构域进行基因本体的标注，首次获得了大规模的领域级功能标注。我们对这些注释与Inter-pro签名的功能注释中隐含的注释进行了大规模比较，结果表明该方法的性能在全局上更好。我们还详细讨论了一些特殊的例子。自动生成并在线提供，该资源可以成为未来手动管理注释的基础。
Most proteins are organized in domains which can be seen as independent modular units in terms of molecular function (MF). Nevertheless, current functional annotations are done on a "whole-chain" basis without associating specific functions to the individual domains. We present here an automatic method for discerning which particular structural domain within a protein is responsible for a given MF originally attributed to the whole protein. By annotating the SCOP structural domains with gene ontology terms using this method, we obtained the first large-scale functional annotation at the domain level. We performed a large-scale comparison of these annotations with the ones implicit in the functional annotations of Inter-pro signatures, showing that the performance of this method is globally better. We also discuss in detail some particular examples. Generated automatically and available online, this resource could be the basis for future manually curated annotations.