The preparation, characterization, and evaluation of cationic microparticles for DNA vaccine delivery
The preparation, characterization, and evaluation of cationic microparticles for DNA vaccine delivery
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DOI：
10.1023/a:1011053931523
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发表时间：
2001-05-01
影响因子：
3.7
通讯作者：
O'Hagan, D
中科院分区：
文献类型：
--
作者：
Briones, M;Singh, M;O'Hagan, D
The use of DNA vaccines has become well established (1), and intramuscular injection has resulted in the induction of potent immune responses, including antibody and cyto-toxic T lymphocytes (CTL)(1, 2). However, although immune responses have been induced in primates, including humans, high doses of DNA on multiple occasions have been required (3—6).Therefore, several approaches are currently under evalu-ation to improve the potency of DNA vaccines, including vector modification to enhance antigen expression (7), physi-cal delivery methods (8, 9), and the use of vaccine adjuvants (10). As an alternative, we have developed cationic microparticles with adsorbed plasmids as a delivery system for DNA vaccines (11), using the biodegradable and biocompatible polymer, polylactide-co-glycolide (PLG)(12). PLG has pre-viously been used for a wide range of biomedical purposes, including the preparation of drug delivery systems (13). Al-though PLG microparticles (14—20) have previously been de-scribed as a delivery system for DNA vaccines, these formulations had some inherent disadvantages over our proposed concept. The entrappment of DNA within the microparticles
