Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus x B. carinata hybrids
Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus x B. carinata hybrids
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DOI：
10.1007/s00122-010-1468-5
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Cowling, Wallace A.
中科院分区：
文献类型：
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作者：
Mason, Annaliese S.;Nelson, Matthew N.;Cowling, Wallace A.
研究了8个甘蓝型油菜（AAC（n）C（n））× B的基因型对同源重组和同源重组频率的影响。carinata（（BBCCc）-C-c）种间杂种（基因组组成C（n）C（c）AB）。通过对67个未减数小孢子衍生后代的微卫星标记分析来评估减数分裂重组事件。34个微卫星标记在64个位点上扩增出83个A、B、C-n和C-c等位基因，其中7个标记扩增出26个等位基因，可用于等位基因拷贝数的测定。杂种后代组间A、B基因组等位基因丢失率差异显著（P < 0.0001），为6 ~ 22%。等位基因拷贝数分析显示19个A-C、3个A-B和10个B-C重复/缺失事件归因于同源重组。此外，检测到55个缺失和19个重复，而没有伴随的剂量变化的同源等位基因。杂交后代组在观察到的A和B基因组以及二倍体C基因组中等位基因的丢失、获得和交换的频率方面各不相同。杂种后代的自交育性随着B基因组位点（而不是A基因组位点）丢失的增加而降低。具有高水平的同源和同源交换的杂交基因型可用于B之间的遗传渐渗。carinata和B.欧洲油菜（卡诺拉）中，并且具有低水平的那些可用于开发稳定的合成芸苔属异源多倍体。
We investigated the influence of genotype on homoeologous and homologous recombination frequency in eight different Brassica napus (AAC(n)C(n)) x B. carinata ((BBCCc)-C-c) interspecific hybrids (genome composition C(n)C(c)AB). Meiotic recombination events were assessed through microsatellite marker analysis of 67 unreduced microspore-derived progeny. Thirty-four microsatellite markers amplified 83 A-, B-, C-n- and C-c-genome alleles at 64 loci, of which a subset of seven markers amplifying 26 alleles could be used to determine allele copy number. Hybrid genotypes varied significantly in loss of A- and B-genome alleles (P < 0.0001), which ranged from 6 to 22% between hybrid progeny sets. Allele copy number analysis revealed 19 A-C, 3 A-B and 10 B-C duplication/deletion events attributed to homoeologous recombination. Additionally, 55 deletions and 19 duplications without an accompanying dosage change in homoeologous alleles were detected. Hybrid progeny sets varied in observed frequencies of loss, gain and exchange of alleles across the A and B genomes as well as in the diploid C genome. Self-fertility in hybrid progeny decreased as the loss of B-genome loci (but not A-genome loci) increased. Hybrid genotypes with high levels of homologous and homoeologous exchange may be exploited for genetic introgressions between B. carinata and B. napus (canola), and those with low levels may be used to develop stable synthetic Brassica allopolyploids.