Proteomics reveals the effects of sustained weight loss on the human plasma proteome
Proteomics reveals the effects of sustained weight loss on the human plasma proteome
复制标题
DOI：
10.15252/msb.20167357
复制
发表时间：
2016-12-01
影响因子：
9.9
通讯作者：
Mann, Matthias
中科院分区：
文献类型：
--
作者：
Geyer, Philipp E.;Albrechtsen, Nicolai J. Wewer;Mann, Matthias
持续减肥是多种代谢状况下的首选干预措施，但对个人健康状况的影响仍然不明确。在这里，我们研究了 43 名肥胖个体的血浆蛋白质组，这些个体经历了 8 周 12% 的体重减轻，随后一年的体重维持。使用基于质谱的血浆蛋白质组分析，我们测量了 1,294 个血浆蛋白质组。对队列的纵向监测显示个体特异性蛋白质水平与减肥对血浆蛋白质组的广泛影响反映在 93 种显着影响的蛋白质中。脂肪细胞分泌的SERPINF1和载脂蛋白APOF1受到最显着的调节，倍数变化分别为-16%和+37%(P < 10(-13))，并且整个载脂蛋白家族表现出特征性差异调节。临床实验室参数反映在血浆蛋白质组中，八种血浆蛋白与胰岛素抵抗的相关性比已知的标记物脂联素更好。几乎所有研究参与者都从蛋白质组数据定义的十种蛋白质炎症组的减肥中受益。我们的结论是，血浆蛋白质组分析广泛评估和监测代谢疾病的干预措施。
Sustained weight loss is a preferred intervention in a wide range of metabolic conditions, but the effects on an individual's health state remain ill-defined. Here, we investigate the plasma proteomes of a cohort of 43 obese individuals that had undergone 8 weeks of 12% body weight loss followed by a year of weight maintenance. Using mass spectrometry-based plasma proteome profiling, we measured 1,294 plasma proteomes. Longitudinal monitoring of the cohort revealed individual-specific protein levels with wide-ranging effects of losing weight on the plasma proteome reflected in 93 significantly affected proteins. The adipocyte-secreted SERPINF1 and apolipoprotein APOF1 were most significantly regulated with fold changes of -16% and +37%, respectively (P < 10(-13)), and the entire apolipoprotein family showed characteristic differential regulation. Clinical laboratory parameters are reflected in the plasma proteome, and eight plasma proteins correlated better with insulin resistance than the known marker adiponectin. Nearly all study participants benefited from weight loss regarding a ten-protein inflammation panel defined from the proteomics data. We conclude that plasma proteome profiling broadly evaluates and monitors intervention in metabolic diseases.