Development of a combined biological and chemical process for production of industrial aromatics from renewable resources.
Development of a combined biological and chemical process for production of industrial aromatics from renewable resources.
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DOI：
10.1146/annurev.micro.61.080706.093248
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发表时间：
2007-09
影响因子：
10.5
通讯作者：
F. Sima Sariaslani
中科院分区：
文献类型：
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作者：
F. Sima Sariaslani
从可再生资源生产工业芳香族化学品可以为传统的化学合成路线提供有竞争力的替代方案。本文综述了利用葡萄糖生产对羟基肉桂酸（pHCA）和对羟基苯乙烯（pHS）的微生物工程。使用过表达真菌苯丙氨酸/酪氨酸解氨酶（PAL）和细菌pHCA脱羧酶（pdc）基因的酪氨酸生产大肠杆菌菌株证明了最初的工艺概念。该生物工艺的进一步开发使得pHCA和pHS生产步骤解偶联，以减轻其对生产宿主的毒性。最后的过程包括发酵步骤，使用酪氨酸过量产生的E. coli菌株。该步骤之后是通过固定化的E.过量表达红酵母PAL基因的大肠杆菌细胞。最后，pHCA的化学脱羧产生pHS。这种多方面的方法，它集成了生物学，化学和工程学，允许在适合工业应用的规模上开发经济的工艺。
Production of industrial aromatic chemicals from renewable resources could provide a competitive alternative to traditional chemical synthesis routes. This review describes the engineering of microorganisms for the production of p-hydroxycinnamic acid (pHCA) and p-hydroxystyrene (pHS) from glucose. The initial process concept was demonstrated using a tyrosine-producing Escherichia coli strain that overexpressed both fungal phenylalanine/tyrosine ammonia lyase (PAL) and bacterial pHCA decarboxylase (pdc) genes. Further development of this bioprocess resulted in uncoupling the pHCA and pHS production steps to mitigate their toxicity to the production host. The final process consists of a fermentation step to convert glucose to tyrosine using a tyrosine-overproducing E. coli strain. This step is followed by a single biotransformation reaction to deaminate tyrosine to pHCA through immobilized E. coli cells that overexpress the Rhodotorula glutinis PAL gene. Finally, chemical decarboxylation of pHCA produces pHS. This multifaceted approach, which integrates biology, chemistry, and engineering, has allowed development of an economical process at scales suitable for industrial applications.