Histomorphometric and Densitometric Changes in the Femora of Spinal Cord Transected Mice
Histomorphometric and Densitometric Changes in the Femora of Spinal Cord Transected Mice
复制标题
脊髓横断小鼠股骨的组织形态学和密度变化
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
P. Guertin
中科院分区：
文献类型：
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作者：
S. Picard;N. Lapointe;Jacques P. Brown;P. Guertin
脊髓损伤（SCI）通常会导致创伤后几个月到几年内显着的骨组织损失。尽管越来越多的大鼠模型数据可用于研究 SCI 相关骨丢失的潜在机制，但人们对 SCI 小鼠模型中骨组织变化的程度和速度知之甚少。目的是定量表征和描述成年截瘫小鼠 1 个月内股骨组织的变化。对在低胸水平（Th9/10）横断脊髓的 3 至 4 个月大的 CD1 小鼠进行组织形态测量和密度测量。我们发现横断后 30 天内骨体积 (-22%)、小梁厚度 (-10%) 和小梁数量 (-14%) 普遍减少。双能 X 射线吸收测量显示骨矿物质密度没有变化，但骨矿物质含量显着降低 (-14%)。这些结果表明，成年小鼠股骨脊髓横断后仅几周内就发生了巨大的结构变化。鉴于用于小鼠研究的遗传和分子研究工具的可用性不断增加，该小鼠模型可能有助于进一步研究与 SCI 相关的脱矿质的细胞和分子机制。 Anat Rec，291:303–307，2008。© 2008 Wiley-Liss, Inc.
Spinal cord injury (SCI) leads generally to significant bone tissue loss within a few months to a few years post–trauma. Although, increasing data from rat models are available to study the underlying mechanisms of SCI‐associated bone loss, little is known about the extent and rapidity of bone tissue changes in mouse models of SCI. The objectives are to characterize and describe quantitatively femoral bone tissue changes during 1 month in adult paraplegic mice. Histomorphometric and densitometric measurements were performed in 3‐ to 4‐month‐old CD1 mice spinal cord transected at the low‐thoracic level (Th9/10). We found a general decrease in bone volume (−22%), trabecular thickness (−10%), and trabecular number (−14%) within 30 days post‐transection. Dual‐energy X‐ray absorptiometric measurements revealed no change in bone mineral density but a significant reduction (−14%) in bone mineral content. These results show large structural changes occurring within only a few weeks post–spinal cord transection in the femora of adult mice. Given the increasing availability of genetic and molecular research tools for research in mice, this murine model may be useful to study further the cellular and molecular mechanisms of demineralization associated with SCI. Anat Rec, 291:303–307, 2008. © 2008 Wiley‐Liss, Inc.
影响因子：
4.1
作者：
Squire, M;Donahue, LR;Judex, S
通讯作者：
Judex, S