Effects of estradiol on prolactin production and dihydroergocryptine-induced inhibition of prolactin production in primary cultures of rat pituitary cells.
Effects of estradiol on prolactin production and dihydroergocryptine-induced inhibition of prolactin production in primary cultures of rat pituitary cells.
复制标题
雌二醇对大鼠垂体细胞原代培养物中催乳素产生的影响和二氢麦角隐亭诱导的催乳素产生抑制。
DOI：
10.1210/endo-106-4-1108
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发表时间：
1980
期刊：
影响因子：
4.8
通讯作者：
P. Dannies
中科院分区：
文献类型：
--
作者：
B. West;P. Dannies
我们使用雄性大鼠垂体前细胞的原代培养来表征17β-雌二醇预处理的两种效果：1)克服二氢麦角隐碱（一种多巴胺激动剂）在培养基中对PRL积累（生产）的抑制的能力，以及2)刺激PRL的产生。含有未经处理血清的培养基含有20 pm雌二醇（E2）。在这种培养基中，与二氢麦角隐碱孵育12或24小时，在150pm产生一半的PRL抑制；最大抑制值降低到控制值的32%。50 nm E2预处理5 d后，二氢麦角隐碱对PRL产生的最大抑制作用降至对照的65%，对麦角菌浓度的影响很小或没有影响，产生半最大抑制作用。此外，50 nm E2预处理并没有增加基础PRL的产量。计算含炭处理血清的培养基中E2含量小于2pm。在该介质中，二氢麦角隐碱引起半极限…
We have used primary cultures of male rat anterior pituitary cells to characterize two effects of pretreatment with 17β-estradiol: 1) the ability to overcome the inhibition of PRL accumulation in the medium (production) by dihydroergocryptine, a dopamine agonist, and 2) the stimulation of PRL production. Medium containing untreated serum contained 20 pm estradiol (E2). In this medium, incubation with dihydroergocryptine for 12 or 24 h caused half-maximal inhibition of PRL production at 150 pm; maximal inhibition reduced values to 32% of the control value. Pretreatment with 50 nm E2 for 5 days decreased the maximal inhibition of PRL production caused by dihydroergocryptine to 65% of control values, with little or no effect on the concentration of ergot which caused half-maximal inhibition. In addition, pretreatment with 50 nm E2 did not increase basal PRL production. Medium containing charcoaltreated serum was calculated to contain less than 2 pm E2. In this medium, dihydroergocryptine caused half-maximal ...