Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139
Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139
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DOI：
10.1111/j.1574-695x.1998.tb01128.x
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发表时间：
1998-03-01
影响因子：
--
通讯作者：
Takeda, Y
中科院分区：
文献类型：
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作者：
Hoshino, K;Yamasaki, S;Takeda, Y
建立了一种多重聚合酶链反应方法，用于同时检测霍乱弧菌O1和O139血清群特异性rfb序列和ctxA特异性序列。多重PCR检测具有较高的特异性和敏感性，每次检测霍乱弧菌O1和O139分别可检测65 cfu和200 cfu。对加尔各答传染病医院121例患者粪便标本进行多重PCR检测，结果表明，以培养法为标准，多重PCR检测的敏感性为100%，特异性为95.2%。除38例PCR阳性粪便标本外，还有4例培养法阴性而PCR检测阳性的标本属于O139血清群。所有38份经PCR检测为O1或O139血清组阳性的粪便样本ctxA扩增子也均为阳性，表明O1和O139霍乱弧菌菌株具有产生霍乱毒素的遗传潜力。(C) 1998年欧洲微生物学会联合会。Elsevier Science B.V.出版
A multiplex polymerase chain reaction assay was developed for concurrent detection of rfb sequences specific for the O1 and the O139 serogroups of Vibrio cholerae and for ctxA specific sequences. The multiplex PCR assay was found to be highly specific and sensitive and was capable of detecting 65 cfu and 200 cfu per assay of V. cholerae O1 and O139, respectively. Evaluation of the multiplex PCR assay using 121 stool samples from patients admitted to the Infectious Diseases Hospital, Calcutta, showed the assay to be 100% sensitive and 95.2% specific when the culture method was taken as the standard, In addition to the 38 PCR positive stool samples, an additional four samples which were negative by culture method but positive by PCR assay belonged to the O139 serogroup. All the 38 stool samples positive for either O1 or O139 serogroup by PCR assay were also positive for the ctxA amplicon indicating that the O1 and O139 V. cholerae strains have the genetic potential of producing cholera toxin. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.