Possibilities of optical imaging of the 99mTc-based radiopharmaceuticals
Possibilities of optical imaging of the 99mTc-based radiopharmaceuticals
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DOI：
10.1117/1.jbo.19.4.046014
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发表时间：
2014-04-01
影响因子：
3.5
通讯作者：
Chekhonin, Vladimir P.
中科院分区：
文献类型：
--
作者：
Kondakov, Anton K.;Gubskiy, Ilya L.;Chekhonin, Vladimir P.
活体光学成像被广泛应用于临床前研究。近年来，由于各种器官的评估依赖于基于~(99m)Tc的放射性药物(RPS)，因此应用光学成像系统对伽马发射同位素进行临床前可视化已成为人们感兴趣的问题。用IVIS Spectrum CTTM光学成像系统研究了99m-TcRPS的体外放射发光，包括高铁酸盐、白蛋白大聚集体、二硫代丁二酸、植酸盐胶体和乙二胺四亚甲基膦酸。还研究了有和没有闪烁材料的植酸胶体在体内的分布，并与常规闪烁成像的结果进行了比较。可见光发射似乎是由于RPS中包含的水和发光团的辐射发光，而不是切伦科夫辐射。微弱的空气发光影响了背景。利用电荷耦合器件光学成像系统可以探测到基于~(99)Tm的示踪剂诱导的流体的辐射发光。辐射发光强度及其光谱分布取决于周围的流体和已知的发光团。因此，在某些情况下，体内光学成像是可能的，但优选使用闪烁体，例如，硼硅酸盐玻璃或锗酸铋。(C)2014年光学仪器工程师学会(SPIE)
In vivo optical imaging is widely used in preclinical studies. Recently, the application of optical imaging systems for preclinical visualization of gamma-emitting isotopes has become of interest since the evaluation of various organs relies on Tc-99m-based radiopharmaceuticals (RPs). In vitro radioluminescence of Tc-99m-based RPs, including pertechnetate, albumin macroaggregates, dimercaptosuccinic acid, phytate colloid, and ethylenediamine tetramethylene phosphonic acid, was studied with IVIS Spectrum CTTM optical imaging system. The distribution of phytate colloid was also studied in vivo with and without scintillating materials and the results were compared with those obtained with a conventional scintigraphy. The visible light emission appeared to be due to the radioluminescence of water and luminophores contained in RPs rather than from Cherenkov radiation. Weak air luminescence affected the background. The radioluminescence of fluids induced by Tc-99m-based tracers could be detected using charge-coupled device optical imaging systems. The radioluminescence intensity and its spectral distribution depend on the surrounding fluid and known luminophores present. Thus, in some cases the in vivo optical imaging is possible but the use of scintillator, e.g., borosilicate glass or bismuth germanate, is preferred. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)