Zika virus infection of adult and fetal STAT2 knock-out hamsters
Zika virus infection of adult and fetal STAT2 knock-out hamsters
复制标题
DOI：
10.1016/j.virol.2017.04.013
复制
发表时间：
2017-07-01
期刊：
影响因子：
3.7
通讯作者：
Julander, Justin G.
中科院分区：
文献类型：
--
作者：
Siddharthan, Venkatraman;Van Wettere, Arnaud J.;Julander, Justin G.
在成年和胎儿STAT2敲除（KO）仓鼠中研究寨卡病毒（ZIKV）感染。成年动物皮下注射ZIKV导致发病率、死亡率和子宫、胎盘、脑、脊髓和睾丸的感染，因此提供了在除小鼠之外的第二啮齿动物物种中评估先天性ZIKV感染的机会。在睾丸中观察到具有支持细胞和精原细胞形态的ZIKV感染细胞，这可能与性传播和雄性不育有关。在性交后8天作为胎儿暴露于ZIKV的新生儿不小于对照。然而，在KO仓鼠的一些但非全部胎盘和胎脑中检测到感染性病毒和ZIKV RNA。STAT2 KO仓鼠可用于解决性传播，发病机制，胎儿感染途径和神经系统疾病的结果，也可用于抗病毒或疫苗研究，以确定干预策略。
Zika virus (ZIKV) infection was investigated in adult and fetal STAT2 knock-out (KO) hamsters. Subcutaneous injection of ZIKV of adults resulted in morbidity, mortality, and infection of the uterus, placenta, brain, spinal cord, and testicles, thus providing an opportunity to evaluate congenital ZIKV infection in a second rodent species besides mice. ZIKV-infected cells with morphologies of Sertoli cells and spermatogonia were observed in the testes, which may have implications for sexual transmission and male sterility. Neonates exposed as fetuses to ZIKV at 8 days post-coitus were not smaller than controls. Nevertheless, infectious virus and ZIKV RNA was detected in some, but not all, placentas and fetal brains of KO hamsters. STAT2 KO hamsters may be useful for addressing sexual transmission, pathogenesis, routes of fetal infection, and neurological disease outcomes, and may also be used in antiviral or vaccine studies to identify intervention strategies.