Aliikangiella marina gen. nov., sp nov., a marine bacterium from the culture broth of Picochlorum sp 122, and proposal of Kangiellaceae fam. nov in the order Oceanospirillales
Aliikangiella marina gen. nov., sp nov., a marine bacterium from the culture broth of Picochlorum sp 122, and proposal of Kangiellaceae fam. nov in the order Oceanospirillales
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Aliikangiella 码头根。
DOI：
10.1099/ijsem.0.000601
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发表时间：
2015-12-01
影响因子：
2.8
通讯作者：
Li, Xiang
中科院分区：
文献类型：
--
作者：
Wang, Guanghua;Tang, Mingxing;Li, Xiang
从海洋微藻Picochloruma sp.122的培养液中分离到一株革兰氏染色阴性、不运动、不产芽孢的长杆状细菌，命名为菌株GYP-15（T）。系统发育分析表明，菌株GYP-15（T）与其近缘种Kangiella aquimarina KCTC 12183（T）具有90.6%的16 S rRNA基因序列相似性，代表了一个由GYP-15（T）与海洋植物目Kangiella属和Pleionea属成员组成的系统发育谱系。化学分类学和生理学特征，包括主要的细胞脂肪酸，NaCl耐受性和碳源利用模式，也可以很容易地将菌株GYP-15（T）与所有已建立的属和种区分开来。因此，可以得出结论，菌株GYP-15（T）代表新属的新种，其名称为Aliikangiella marina gen. nov.，sp. nov.的报道。模式菌株为GYP-15（T）（=MCCC 1 K 01163（T）=KCTC 42667（T））。根据系统发育分析结果、16 S rRNA基因序列特征和一些生理特性，将Kangiella、Pleionea和Aliikangiella三个属划分为一个新的科，即Kangiellaceae fam.十一月，属于海洋藻目。
A Gram-stain-negative, non-motile, non-spore-forming, long rod-shaped bacterium, designated strain GYP-15(T), was isolated from the culture broth of a marine microalga, Picochloruma sp. 122. Phylogenetic analyses revealed that strain GYP-15(T) shared 90.6 % 16S rRNA gene sequence similarity with its closest relative, Kangiella aquimarina KCTC 12183(T), and represents a distinct phylogenetic lineage in a robust Glade consisting of GYP-15(T) and members of the genera Kangiella and Pleionea in the order Oceanospirillales. Chemotaxonomic and physiological characteristics, including major cellular fatty acids, NaCI tolerance-and pattern of carbon source utilization, could also readily distinguish strain GYP-15(T) from all established genera and species. Thus, it is concluded that strain GYP-15(T) represents a novel species of a new genus, for which the name Aliikangiella marina gen. nov., sp. nov. is proposed. The type strain of Aliikangiella marina is GYP-15(T) (=MCCC 1K01163(T)=KCTC 42667(T)). Based on phylogenetic results, 16S rRNA gene signature nucleotide pattern and some physiological characteristics, the three genera Kangiella, Pleionea and Aliikangiella are proposed to make up a novel family, Kangiellaceae fam. nov., in the order Oceanospirillales.