Novel quantitative real-time PCR approach to determine safflower (Carthamus tinctorius) adulteration in saffron (Crocus sativus).
Novel quantitative real-time PCR approach to determine safflower (Carthamus tinctorius) adulteration in saffron (Crocus sativus).
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DOI：
10.1016/j.foodchem.2017.02.136
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发表时间：
2017-08
期刊：
影响因子：
8.8
通讯作者：
C. Villa;J. Costa;M. B. Oliveira;I. Mafra
中科院分区：
文献类型：
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作者：
C. Villa;J. Costa;M. B. Oliveira;I. Mafra
This work intended to develop DNA-based methods to detect and quantify safflower as an adulterant in saffron. Species-specific PCR and real-time PCR with EvaGreen dye targeting the ITS region ofCarthamus tinctoriusL. (safflower) were successfully proposed. The assays allowed absolute and relative sensitivities of 2 pg of safflower DNA (∼1.4 DNA copies) and 0.1% of safflower in saffron (Crocus sativusL.), respectively. A normalised real-time PCR approach was also proposed in the range of 0.1–20% (w/w) of safflower in saffron, which was successfully validated and applied to commercial saffron samples (stigmas, powders and seasonings). From 19 samples, three were positive to safflower, though at levels below the limit of detection, suggesting cross-contamination rather than adulteration. In this work, specific, sensitive and accurate tools were proposed to authenticate saffron. To the best of our knowledge, this is the first successful attempt to quantify safflower by a DNA-based approach.
