DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING
DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING
复制标题
DOI：
10.1016/0925-4005(95)85018-x
复制
发表时间：
1995-03-01
影响因子：
8.4
通讯作者：
TURNER, APF
中科院分区：
文献类型：
--
作者：
CAGNINI, A;PALCHETTI, I;TURNER, APF
本文报道了一种基于丝网印刷电极的一次性胆碱生物传感器的制备及其在有机磷农药和氨基甲酸酯类农药检测中的应用。在活性炭上混合0.5%钌以形成用于工作电极表面的简单石墨基油墨，增加了对过氧化氢的敏感性。胆碱生物传感器是基于这样的电极耦合与胆碱氧化酶的吸附固定。利用该生物传感器检测了氨基甲酸酯类和有机磷类农药对乙酰胆碱酯酶的抑制作用。使用所述的优化程序（pH值，缓冲液组成，孵育时间，底物浓度），我们能够检测低至1 nM的农药（克百威）浓度。
A disposable choline biosensor based on ruthenized carbon screen-printed electrodes has been prepared and its use for monitoring organophosphorus pesticides and carbamates is described. The presence of 0.5% ruthenium on activated carbon mixed to form a simple graphite-based ink for the working electrode surface increased the sensitivity towards hydrogen peroxide. The choline biosensor is based on such an electrode coupled with choline oxidase immobilized by adsorption. The inhibition effect of carbamates and organophosphorus pesticides on acetylcholinesterase was detected using this biosensor. With the optimized procedure described (pH, buffer composition, incubation time, substrate concentration) we are able to detect concentrations of pesticides (carbofuran) as low as 1 nM.