The effect of temperature and pH on the growth of lactic acid bacteria: a pH-auxostat study
The effect of temperature and pH on the growth of lactic acid bacteria: a pH-auxostat study
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DOI：
10.1016/s0168-1605(02)00537-8
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发表时间：
2003-08-15
影响因子：
5.4
通讯作者：
Paalme, T
中科院分区：
文献类型：
--
作者：
Adamberg, K;Kask, S;Paalme, T
唾液链球菌唾液亚种的生长。嗜热乳球菌（Lactococcus lactis subsp.）乳酸菌、德氏乳杆菌乳酸亚种（Lactobacillus delbrueckii subsp.）保加利亚乳杆菌、嗜酸乳杆菌和副干酪乳杆菌在恒pH培养条件下进行了研究。采用计算机控制pH或温度的平滑变化，研究pH和温度对培养特性（比生长速率It、生长产量Y-ATP和比乳酸生产速率Q（ATP））的影响。mu和Q（ATP）随温度升高的行为表明，ATP生产能力是决定乳酸菌（LAB）最大生长速率的重要因素。随着温度的升高，Y-ATP的减少导致比生长速率的降低，而Q（ATP）保持不变甚至增加。随着pH值的降低，Y-ATP和Q（ATP）均降低。S.嗜热菌St20在44 ℃时的最大比生长速率为2.2h（-1），是最酸敏感的菌株。洛杉矶具有最低mu（max）的副干酪E1H3是对pH变化最耐受的菌株。对L.保加利亚S.嗜热菌在牛奶中的pH-恒流器与pH-降低的纯培养实验一致。研究表明，pH-温度平稳可控的恒pH培养法是乳酸菌工艺特性测定和比较生理研究的有效方法。(C)2002 Elsevier Science B.V.保留所有权利。
The growth of Streptococcus salivarius subsp. thermophilus, Lactococcus lactis subsp. lactis, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus and Lactobacillus paracasei was studied in a pH-auxostat. The computer-controlled smooth change of pH or temperature was used to study the effect of pH and temperature on the culture characteristics (specific growth rate It, growth yield Y-ATP and specific lactate production rate Q(ATP)). The behaviour of mu and Q(ATP) with an increase of temperature suggested that ATP production capacity was an important factor in determining the maximum growth rate of lactic acid bacteria (LAB). The decrease of Y-ATP with increase of temperature resulted in the decrease of specific growth rate while Q(ATP) remained constant or even increased. With the decrease of pH, decrease of both Y-ATP and Q(ATP) was observed. S. thermophilus St20 having the highest maximum specific growth rate of 2.2 h(-1) at 44 degreesC was the most acid sensitive strain. The L. paracasei E1H3 having the lowest mu(max) was the most tolerant strain to pH change. The behaviour of mixed culture of L. bulgaricus and S. thermophilus in milk in a pH-auxostat with pH-decrease was in agreement with the pure culture experiments of the same species. The study showed that pH-auxostat with smooth controlled change of pH and temperature is an effective method for determination of technological characteristics and comparative physiological study of LAB. (C) 2002 Elsevier Science B.V. All rights reserved.