Glucose-dependent glycosylation of secretory glycoprotein in mouse myeloma cells.
Glucose-dependent glycosylation of secretory glycoprotein in mouse myeloma cells.
复制标题
小鼠骨髓瘤细胞中分泌糖蛋白的葡萄糖依赖性糖基化。
DOI：
10.1016/0003-9861(79)90131-0
复制
发表时间：
1979
影响因子：
3.9
通讯作者：
E. Heath
中科院分区：
文献类型：
--
作者：
N. J. Stark;E. Heath
小鼠骨髓瘤肿瘤MOPC-46产生κ型免疫球蛋白轻链，其可从荷瘤动物的尿液中分离。该蛋白质具有单个碳水化合物侧链，通过葡糖胺键与天冬酰胺残基28连接。当肿瘤的活单细胞悬液在含有丙酮酸钠作为碳源和能量源的体外最低必需培养基中孵育时，合成和分泌的主要蛋白质对应于非糖基化形式的κ轻链。然而，当葡萄糖或甘露糖取代丙酮酸钠作为碳源时，免疫球蛋白轻链以完全糖基化的天然形式合成和分泌。蛋白质的正常糖基化对培养基中葡萄糖或甘露糖存在的依赖性对于这些化合物是相对特异性的，因为用果糖、半乳糖、甘油、核糖或N-乙酰葡糖胺取代是无效的。在丙酮酸钠存在下产生的非糖基化蛋白通过免疫沉淀和凝胶电泳表征为非糖基化MOPC-46轻链。当孵育混合物中含有衣霉素时，肿瘤细胞在葡萄糖存在下产生相同的非糖基化蛋白。
The mouse myeloma tumor, MOPC-46, produces a kappa-type immunoglobulin light chain that may be isolated from the urine of tumor-bearing animals. This protein possesses a single carbohydrate side chain, attached by glycosylamine linkage to asparagine residue 28. When viable single cell suspensions of the tumor are incubatedin vitroin minimum essential medium containing sodium pyruvate as a source of carbon and energy, the major protein synthesized and secreted corresponds to a nonglycosylated form of the kappa light chain. However, when glucose or mannose are substituted for sodium pyruvate as a source of carbon, the immunoglobulin light chain is synthesized and secreted in the fully glycosylated, native form. The dependence of normal glycosylation of the protein on the presence of either glucose or mannose in the medium is relatively specific for these compounds since substitution with either fructose, galactose, glycerol, ribose, orN-acetylglucosamine was ineffective. The nonglycosylated protein produced in the presence of sodium pyruvate was characterized as nonglycosylated MOPC-46 light chain by immunoprecipitation and gel electrophoresis. An identical nonglycosylated protein was produced by tumor cells in the presence of glucose when the incubation mixtures contained tunicamycin.