Flow cytochemical patterns of white blood cells in human haematopoietic malignancies
Flow cytochemical patterns of white blood cells in human haematopoietic malignancies
复制标题
人类造血系统恶性肿瘤白细胞的流式细胞化学模式
DOI：
10.1111/j.1365-2141.1987.tb06886.x
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发表时间：
1987
影响因子：
6.5
通讯作者：
J. Trujillo
中科院分区：
文献类型：
--
作者：
B. Drewinko;P. Bollinger;C. Brailas;S. Moyle;J. Wyatt;E. Simson;D. Johnston;J. Trujillo
使用Technicon H-6000自动血液分析仪测量了118例急性白血病患者（68例未经治疗; 50例经治疗）的外周血样本。该仪器除了测量经典血液学参数（即细胞计数、血红蛋白浓度等）外，通过流式细胞化学（过氧化物酶含量）和体积（光散射）鉴别对104 WBC进行分类计数。不考虑RBC和血小板计数及其体积分布特征，白血病疾病最重要的诊断参数是WBC计数、WBC分类计数、通过自动分类计数获得的大未染色细胞（LUC）和高过氧化物酶（HPX）细胞的比例以及WBC过氧化物酶含量分布（MPA）的平均值。粒细胞白血病的MPA低于正常，淋巴细胞白血病的MPA值高于正常。M1白血病的特征还在于大比例的LUC和低分数的HPX，而M2白血病显示低LUC和高HPX。M3白血病LUC低，HPX高。M4白血病有较大的LUC和“单核细胞”成分和中等比例的HPX。M5白血病有非常大量的LUC、“单核细胞”和“淋巴细胞”，以及正常的HPX。对于M1白血病，诱导治疗后LUC低于7%与化疗诱导的正常细胞形态学变化相关，而LUC高于10%通常表明诱导失败与残留原始细胞相关。如果治疗成功，M2和M3白血病的特征性HPX群体减少。我们研究的所有M4白血病均未能进入缓解期，并继续显示高比例的HPX和LUC。同样，大多数M5白血病对治疗的反应较差，并且总是显示出非常高的LUC比例。未经治疗的淋巴细胞白血病表现出高LUC，正常HPX和高比例的“淋巴细胞”。毛细胞白血病表现出几乎相等比例的“淋巴细胞”和LUC。所有淋巴细胞白血病实体的成功化疗与LUC的快速下降相关。“淋巴细胞”的缓慢减少和HPX的中度和短暂增加。因此，流式细胞化学不仅可以帮助分离急性白血病沿着与FAB分类与非形态学标准，而且在后续的患者与这些疾病。
Peripheral blood samples from 118 patients with acute leukaemia (68 untreated; 50 treated) were measured with the Technicon H‐6000 automated haematology analyser. This instrument provides, in addition to measurements of the classical haematology parameters (i.e. cell counts, haemoglobin concentration, etc.), a differential count on 104 WBC effected by means of flow cytochemistry (peroxidase content) and volume (light scatter) discrimination. Disregarding RBC and platelet counts and their volume distribution profiles, the most important diagnostic parameters for leukaemic disease were the WBC count, the WBC differential count, and the proportions of large unstained cells (LUC) and high peroxidase (HPX) cells obtained by the automated differential count as well as the mean value of the WBC peroxidase content distribution (MPA). Granulocytic leukaemias had lower MPA than normal and lymphocytic leukaemias had MPA values above normal. M1 leukaemias were also characterized by large proportions of LUC and low fractions of HPX, while M2 leukaemias showed low LUC with high HPX. M3 leukaemias had low LUC and very high HPX. M4 leukaemias had large LUC and‘monocytic’components and a modest fraction of HPX. M5 leukaemias had very large numbers of LUC,‘monocytes’and‘lymphocytes’and a normal HPX. For M1 leukaemia, the presence of less than 7% LUC following induction treatment was related to morphological changes of normal cells induced by chemotherapy while LUC above 10% usually indicated unsuccessful induction associated with the presence of residual blasts. If treatment was successful, M2 and M3 leukaemias characteristically decreased their HPX population. All M4 leukaemias studied by us failed to enter remission and continued to display high proportions of HPX and LUC. Similarly, most M5 leukaemias had a poor response to treatment and always showed a very high proportion of LUC. Untreated lymphocytic leukaemias demonstrated high LUC, normal HPX and a high proportion of‘lymphocytes’. Hairy cell leukaemias showed almost equal proportions of‘lymphocytes’and LUC. Successful chemotherapy of all lymphoid leukaemia entities was associated with rapid decreases in LUC. slower decrements of‘lymphocytes’and moderate and transient increments in HPX. Thus, flow cytochemistry can assist not only in the segregation of acute leukaemias along with FAB classification with nonmorphologic criteria, but also in the follow up of patients with these diseases.