Drought-induced expression of aquaporin genes in leaves of two common bean cultivars differing in tolerance to drought stress
Drought-induced expression of aquaporin genes in leaves of two common bean cultivars differing in tolerance to drought stress
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DOI：
10.1007/s10265-017-0920-x
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发表时间：
2017-03
影响因子：
2.8
通讯作者：
M. Zupin;A. Sedlar;M. Kidrič;V. Meglič
中科院分区：
文献类型：
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作者：
M. Zupin;A. Sedlar;M. Kidrič;V. Meglič
水通道蛋白是菜豆（Phaseolus vulgarisL.）干旱影响这一重要作物的产量和质量。为了更好地理解水孔蛋白在菜豆中的作用，在两个品种中测定了干旱诱导的几种水孔蛋白的基因表达，更耐旱的台伯和不太耐旱的Starozagorski čern。这两个菜豆品种是从16个欧洲基因型中选出的，其耐旱性是由植物在停止灌溉后枯萎所需的时间和收获时的产量决定的。采用RT-qPCR方法，对3种干旱胁迫、复水和对照条件下2个品种21日龄植株叶片中两种质膜内在蛋白PvPIP 1; 2和PvPIP 2;7以及两种液泡膜内在蛋白PvTIP 1; 1和PvTIP 4; 1的表达模式进行了研究。在干旱胁迫下，所有四种检测的水通道蛋白的基因表达下调。复水后，它恢复到对照植物的水平，甚至更高。PvPIP 2; 7和PvTIP 1; 1在干旱和复水期间的反应尤为明显。PvPIP 2基因的表达; 7和PvTIP 4; 1干旱期间这两种水通道蛋白的表达具有品种特异性，在耐旱的台伯种中下调幅度更大。在干旱胁迫下，台伯植物叶片的相对含水量和水势均高于Starozagorski植物。这些生理参数的差异表明，更大的防止水分流失在台伯河在干旱期间，这可能与水通道蛋白的快速和充分的下调。这些结果表明，植物在干旱胁迫下保持水分的能力涉及到特定水通道蛋白基因表达的及时和充分的下调。
Aquaporin proteins are part of the complex response of common bean (Phaseolus vulgarisL.) to drought which affects the quality and quantity of yield of this important crop. To better understand the role of aquaporins in common bean, drought-induced gene expression of several aquaporins was determined in two cultivars, the more drought tolerant Tiber and the less tolerant Starozagorski čern. The two bean cultivars were selected among 16 European genotypes based on the tolerance to drought determined by time needed for plants to wilt after withholding irrigation and yield at harvest. The expression patterns of two plasma membrane intrinsic proteins,PvPIP1;2andPvPIP2;7, and two tonoplast intrinsic proteins,PvTIP1;1andPvTIP4;1in leaves of 21 day old plants were determined by RT-qPCR in both cultivars under three degrees of drought stress, and under rehydration and control conditions. Gene expression of all four examined aquaporins was down-regulated in drought stressed plants. After rehydration it returned to the level of control plants or was even higher. The responses ofPvPIP2;7andPvTIP1;1during drought and rehydration were particularly pronounced. The gene expression ofPvPIP2;7andPvTIP4;1during drought was cultivar specific, with greater down-regulation of these two aquaporins in drought tolerant Tiber. Under drought stress the relative water content and water potential of leaves were higher in Tiber than in Starozagorski plants. The differences in these physiological parameters indicate greater prevention of water loss in Tiber during drought, which may be associated with rapid and adequate down-regulation of aquaporins. These results suggest that the ability of plants to conserve water during drought stress involves timely and sufficient down-regulation of gene expression of specific aquaporins.