Investigation of Antimicrobial and Cytotoxic Properties and Specification of Silver Nanoparticles (AgNPs) Derived From Cicer arietinum L. Green Leaf Extract.
Investigation of Antimicrobial and Cytotoxic Properties and Specification of Silver Nanoparticles (AgNPs) Derived From Cicer arietinum L. Green Leaf Extract.
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金鸡儿绿叶提取物银纳米粒(AgNPs)的抗菌和细胞毒性特性及规格研究
DOI：
10.3389/fbioe.2022.855136
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发表时间：
2022
影响因子：
5.7
通讯作者：
Eftekhari A
中科院分区：
文献类型：
--
作者：
Baran A;Fırat Baran M;Keskin C;Hatipoğlu A;Yavuz Ö;İrtegün Kandemir S;Adican MT;Khalilov R;Mammadova A;Ahmadian E;Rosić G;Selakovic D;Eftekhari A
使用生物材料合成金属纳米粒子已成为研究人员经常首选的方法。这种合成方法既快速又便宜。在这项研究中，使用从鹰嘴豆 (Cicer arietinum L.) (CA) 叶子中获得的水提取物来合成银纳米颗粒 (AgNP)。对于合成的 AgNP 的规格，使用了紫外可见分光光度计、傅里叶变换红外光谱 (FT-IR)、X 射线衍射分析 (XRD)、透射电子显微镜 (TEM)、扫描电子显微镜 (SEM)、电子色散 X 射线 (EDX) 和 zeta 电位 (ZP) 分析数据。生物合成的 AgNPs 在 3 小时后表现出最大表面等离子共振为 417.47 nm。通过粉末 XRD 模型，纳米粒子的平均晶粒尺寸确定为 12.17 毫米，具有立方结构。根据 TEM 结果，发现所获得的银纳米粒子的尺寸为 6.11–9.66 nm。 AgNP 表面电荷的 ZP 测量为 -19.6 mV。采用最小抑制浓度（MIC）法测定AgNPs对食品致病菌和酵母菌的抑制效果。 AgNP 在低浓度下表现出高效抑制作用，尤其是对枯草芽孢杆菌 (0.0625) 和金黄色葡萄球菌 (0.125) 菌株的生长。揭示了银纳米粒子对癌细胞系（CaCo-2、U118、Sk-ov-3）和健康细胞系（HDF）的细胞毒性作用。尽管用于对抗癌性和健康细胞系的 AgNP 有所增加，但没有检测到活力百分比显着下降。
Using biological materials to synthesize metallic nanoparticles has become a frequently preferred method by researchers. This synthesis method is both fast and inexpensive. In this study, an aqueous extract obtained from chickpea (Cicer arietinum L.) (CA) leaves was used in order to synthesize silver nanoparticles (AgNPs). For specification of the synthesized AgNPs, UV-vis spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), electron dispersive X-ray (EDX), and zeta potential (ZP) analyses data were used. Biologically synthesized AgNPs demonstrated a maximum surface plasmon resonance of 417.47 nm after 3 h. With the powder XRD model, the mean crystallite dimension of nanoparticles was determined as 12.17 mm with a cubic structure. According to the TEM results, the dimensions of the obtained silver nanoparticles were found to be 6.11–9.66 nm. The ZP of the electric charge on the surface of AgNPs was measured as −19.6 mV. The inhibition effect of AgNPs on food pathogen strains and yeast was determined with the minimum inhibition concentration (MIC) method. AgNPs demonstrated highly effective inhibition at low concentrations especially against the growth of B. subtilis (0.0625) and S. aureus (0.125) strains. The cytotoxic effects of silver nanoparticles on cancerous cell lines (CaCo-2, U118, Sk-ov-3) and healthy cell lines (HDF) were revealed. Despite the increase of AgNPs used against cancerous and healthy cell lines, no significant decrease in the percentage of viability was detected.