METABOLIC ENGINEERING OF MEDICINAL-PLANTS - TRANSGENIC ATROPA-BELLADONNA WITH AN IMPROVED ALKALOID COMPOSITION
METABOLIC ENGINEERING OF MEDICINAL-PLANTS - TRANSGENIC ATROPA-BELLADONNA WITH AN IMPROVED ALKALOID COMPOSITION
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DOI：
10.1073/pnas.89.24.11799
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发表时间：
1992-12-15
影响因子：
11.1
通讯作者：
YAMADA, Y
中科院分区：
文献类型：
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作者：
YUN, DJ;HASHIMOTO, T;YAMADA, Y
莨菪碱是一种重要的抗胆碱能药物，存在于几种茄科植物中。山莨菪碱6 -羟化酶（EC 1.14.11.11）在山莨菪碱合成途径中催化氧化反应。利用农杆菌介导的转化体系，将花菜花叶病毒35S启动子控制下的黑山霉羟化酶基因导入到富含山霉胺的颠茄中。首先通过筛选卡那霉素抗性，然后用羟化酶特异性抗体对叶片样品进行免疫筛选，选择了具有组成性和强表达转基因的转基因植物。在初代转化及其遗传转基因的自生后代中，叶和茎的生物碱含量几乎全部为东莨菪碱。这种经过代谢工程改造的植物将被证明是获得改良药用成分的有用育种材料。
The tropane alkaloid scopolamine is a medicinally important anticholinergic drug present in several solanaceous plants. Hyoscyamine 6beta-hydroxylase (EC 1.14.11.11) catalyzes the oxidative reactions in the biosynthetic pathway leading from hyoscyamine to scopolamine. We introduced the hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system. A transgenic plant that constitutively and strongly expressed the transgene was selected, first by screening for kanamycin resistance and then by immunoscreening leaf samples with an antibody specific for the hydroxylase. In the primary transformant and its selfed progeny that inherited the transgene, the alkaloid contents of the leaf and stem were almost exclusively scopolamine. Such metabolically engineered plants should prove useful as breeding materials for obtaining improved medicinal components.