The α- and β-expansin and xyloglucan endotransglucosylase/hydrolase gene families of wheat:: Molecular cloning, gene expression, and EST data mining
The α- and β-expansin and xyloglucan endotransglucosylase/hydrolase gene families of wheat:: Molecular cloning, gene expression, and EST data mining
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DOI：
10.1016/j.ygeno.2007.06.012
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发表时间：
2007-10-01
期刊：
影响因子：
4.4
通讯作者：
Zhang, Aimin
中科院分区：
文献类型：
--
作者：
Liu, Yong;Liu, Dongcheng;Zhang, Aimin
膨胀蛋白和木葡聚糖葡糖基转移酶/水解酶（XTH）是细胞外蛋白家族，其成员已被证明在细胞壁生长中起重要作用。在这项研究中，三个，六个，和五个成员的小麦a-扩展蛋白（TaEXPA 1至TaEXPA 3），β-扩展蛋白（TaEXPB 1至TaEXPB 6），和XTH（TaXTH 1至TaXTH 5）基因家族，分别从一个矮秆小麦系分离。通过实时RT-PCR进行的mRNA表达分析表明，这些基因在不同的阶段/器官/处理中显示不同的转录水平，这可能表明它们在细胞壁扩展过程中的功能作用。此外，表达水平的比较揭示了大多数扩展蛋白显示出比XTH更低的表达。最后，我们提出了小麦α-和β-expansins和XTH家族的表达序列标签数据挖掘分析。(C)2007爱思唯尔公司All rights reserved.
Expansins and xyloglucan enclotransglucosylase/hydrolases (XTHs) are families of extracellular proteins with members that have been shown to play an important role in cell wall growth. In this study, three, six, and five members of the wheat a-expansin (TaEXPA1 to TaEXPA3), beta-expansin (TaEXPB1 to TaEXPB6), and XTH (TaXTH1 to TaXTH5) gene families, respectively, were isolated from a dwarf wheat line. The mRNA expression analysis by real-time RT-PCR indicates that these genes display different transcription levels in different stages/organs/treatments, possibly suggesting their functional roles in the cell wall expansion process. Moreover, the comparison of the expression levels reveals that most of the expansins show lower expression than the XTHs. Finally, we present the analysis of wheat alpha- and beta-expansins and XTH families by expressed sequence tag data mining. (C) 2007 Elsevier Inc. All rights reserved.