Expression of Thy-1 antigen is not limited to T cells in cultures of mouse hemopoietic cells.
Expression of Thy-1 antigen is not limited to T cells in cultures of mouse hemopoietic cells.
复制标题
Thy-1 抗原的表达不限于小鼠造血细胞培养物中的 T 细胞。
DOI：
10.1073/pnas.79.13.4161
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Scollay,R
中科院分区：
文献类型：
--
作者：
Schrader,JW;Battye,F;Scollay,R
大量的thy -1阳性细胞在骨髓细胞的培养中被观察到，这些骨髓细胞已经耗尽了T细胞，在由豆豆蛋白a激活的脾细胞调节的培养基中生长了3到4天。使用荧光激活细胞分选器分离出具有与胸腺细胞相当的Thy-1水平的细胞。虽然许多是大细胞，但thy -1阳性细胞在t细胞生长因子和豆豆蛋白A的作用下未能生长；然而，大约三分之一的细胞在刺激造血祖细胞的因素存在下增殖。此外，thy -1阳性群体包括能够在琼脂中形成巨噬细胞和粒细胞大集落的细胞，以及在致死辐照小鼠中形成脾脏集落的细胞。thy -1阳性细胞的出现与t细胞生长因子或t细胞衍生的粒细胞/巨噬细胞集落刺激因子的存在无关。这些结果表明，Thy-1可发生在多种小鼠造血干细胞、祖细胞和髓细胞上；它们-1不再被认为是对t细胞谱系承诺的明确标记。
Large numbers of Thy-1-positive cells were observed in cultures of bone marrow cells that had been depleted of T cells and grown for 3 to 4 days in the presence of medium conditioned by concanavalin A-activated spleen cells. Cells bearing levels of Thy-1 comparable with those on the bulk of thymocytes were isolated by using the fluorescence-activated cell sorter. Although many were large blasts, the Thy-1-positive cells failed to grow in response to T-cell growth factor and concanavalin A; about one-third, however, proliferated in the presence of factors stimulating hemopoietic progenitor cells. Furthermore, the Thy-1-positive population included cells capable of forming large colonies of macrophages and granulocytes in agar and cells forming splenic colonies in lethally irradiated mice. The appearance of the Thy-1-positive cells did not correlate with the presence of either T-cell growth factor or T-cell-derived granulocyte/macrophage colony-stimulating factor. These findings indicate that Thy-1 can occur on various murine hemopoietic stem and progenitor cells and myeloid cells; Thy-1 can no longer be regarded as an unambiguous marker of commitment to the T-cell lineage.