Morphological characterization and optimization of conditions for conidial production of Elsinoe ampelina, the causal organism of grapevine anthracnose
Morphological characterization and optimization of conditions for conidial production of Elsinoe ampelina, the causal organism of grapevine anthracnose
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葡萄炭疽病病原微生物 Elsinoe ampelina 的形态特征和分生孢子生产条件优化
DOI：
10.1111/jph.12702
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发表时间：
2018
影响因子：
1.5
通讯作者：
Wang Xiping
中科院分区：
文献类型：
--
作者：
Li Zhi;Dang Huan;Yuan Xiao;He Jingyuan;Hu Zhiqi;Wang Xiping
葡萄炭疽病是由痂囊腔菌引起的一种严重危害葡萄生产的病害。研究了通气、温度、光照和预培养时间等因素对葡萄酒球菌产孢、分生孢子萌发和毒力的影响。殖民地培养基上的菌落形态较PDA瓶上的菌落形态多样。对不同培养方法、温度、光照条件和预培养期的评估表明，在PDA瓶中于21°C黑暗中生长24小时的25天龄菌落产生最佳分生孢子。在此条件下，PDA瓶中的培养物始终产生约5.0 × 106个分生孢子。当培养物在黑暗中保持在25°C时，不发生分生孢子形成。在25°C下24 h后的最高发芽率为80%，而在17°C下12 h后没有观察到发芽。致病性试验表明，接种后4天，葡萄叶片上即可观察到病害症状。红色地球仪。感染后8天，在病斑上观察到新的分生孢子。本研究为产孢提供了一种有效的方法，也可应用于其他痂囊腔菌的产孢。
Grape anthracnose, which is caused byElsinoë ampelina, is a disease that negatively affects grape production. This study aimed to investigate the effects of aeration, temperature, light, and preculture period on the formation ofE.ampelinaconidia and conidial germination and virulence. The colony morphology on potato dextrose agar (PDA) plates was more diverse than that in PDA bottles. The assessment of different culture methods, temperatures, light conditions, and preculture periods revealed that optimal conidial production occurred on 25‐day‐old colonies grown in PDA bottles at 21°C for 24 hr in the dark. The cultures in PDA bottles consistently produced approximately 5.0 × 106conidia under these conditions. No conidial formation occurred when the cultures were kept at 25°C in the dark. The highest germination rate ofE. ampelinawas 80% at 25°C after 24 hr, whereas no germination was observed at 17°C after 12 hr. Pathogenicity tests revealed that symptoms of the disease were observed 4 days postinoculation (dpi) on leaves ofVitis viniferacv. Red Globe. New conidia were observed on the lesions at 8 dpi. This study provides an effective method for the conidial production ofE. ampelinathat may also be applicable for otherElsinoëfungal species.