Fluorogenic hand-held immunoassay for the identification of ricin: Rapid analyte measurement platform
Fluorogenic hand-held immunoassay for the identification of ricin: Rapid analyte measurement platform
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DOI：
10.1080/15321810701454730
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Thompson, H. G.
中科院分区：
文献类型：
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作者：
Fulton, R. E.;Thompson, H. G.
对快速分析物测量平台（RAMPTM）荧光手持式免疫分析（HHA）的包容性/灵敏度、排他性/特异性、样品基质效应、耐用性/稳定性和检测蓖麻毒素（RCA 60）的重现性进行了评价。RCA 60的HHA检测限为14 ng/mL或约140 pg/试验。HHA对蓖麻毒素RCA 60、RCA 120、蓖麻毒素A链和蓖麻毒素B链的检测具有包容性，但对RCA 60和其他毒素的鉴别具有排他性。检测的样品基质均不影响测定性能。RAMPTM比率容许样品体积增加，然而，样品体积减少25%导致读数显著增加。RAMPTM读数在批次间、试剂盒间、日常间和读数员间具有高度重现性。
Rapid analyte measurement platform (RAMPTM) fluorogenic hand-held immunoassays (HHAs) were evaluated for inclusivity/sensitivity, exclusivity/specificity, sample matrix effects, ruggedness/stability, and reproducibility in detection of ricin (RCA60), a potential biological threat agent. The limit of detection of HHAs for RCA60 was 14 ng/mL or approximately 140 pg/test. HHAs were inclusive in detection of ricin RCA60, RCA120, ricin A chain, and ricin B chain and exclusive in discrimination of RCA60 from other toxins. None of the sample matrices tested affected assay performance. RAMPTM ratios were tolerant of increases in sample volume, however, a decrease in sample volume of 25% resulted in significantly increased readings. RAMPTM readings were highly reproducible lot- to-lot,cartridge-to-cartridge, day-to-day, and reader-to-reader.