FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides
FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides
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FvSet2 调节轮枝镰刀菌的真菌生长、致病性和次生代谢
DOI：
10.1016/j.fgb.2017.07.007
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发表时间：
2017-10-01
影响因子：
3
通讯作者：
Gao, Xuewen
中科院分区：
文献类型：
--
作者：
Gu, Qin;Wang, Zhenzhong;Gao, Xuewen
在大多数真核细胞中，组蛋白H3赖氨酸36甲基化(H3K36me)通常与基因表达的激活有关。然而，H3K36me在丝状真菌中的功能在很大程度上是未知的。Set2是酿酒酵母中唯一负责H3K36甲基化的赖氨酸组蛋白甲基转移酶(KHMTase)。在目前的研究中，我们在Fusariwn verticilliodes中发现了酿酒酵母Set2的单一同源基因。我们报告FvSet2负责H3K36(H3K36me3)的三甲基化。FvSET2缺失突变体(Delta FvSet2)在营养生长、FB1生物合成、色素沉着和真菌毒力等方面存在明显缺陷。此外，H3K36的三甲基化对于参与FB1和bikverin生物合成的基因的活跃转录是重要的。这些数据表明，FvSet2在调节轮纹病菌的次生代谢、营养生长和真菌毒力方面起着重要作用。
Histone H3 lysine 36 methylation (H3K36me) is generally associated with activation of gene expression in most eukaryotic cells. However, the function of H3K36me in filamentous fungi is largely unknown. Set2 is the sole lysine histone methyltransferase (KHMTase) enzyme responsible for the methylation of H3K36 in Saccharomyces cerevisiae. In the current study, we identified a single ortholog of S. cerevisiae Set2 in Fusariwn verticillioides. We report that FvSet2 is responsible for the trimethylation of H3K36 (H3K36me3). The FvSET2 deletion mutant (Delta FvSet2) showed significant defects in vegetative growth, FB1 biosynthesis, pigmentation, and fungal virulence. Furthermore, trimethylation of H3K36 was found to be important for active transcription of genes involved in FB1 and bikaverin biosyntheses. These data indicate that FvSet2 plays an important role in the regulation of secondary metabolism, vegetative growth and fungal virulence in F. verticillioides.