Immunohistochemical and biomolecular identification of melatonin 1a and 1b receptors in rat vestibular nuclei
Immunohistochemical and biomolecular identification of melatonin 1a and 1b receptors in rat vestibular nuclei
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DOI：
10.1016/j.anl.2011.09.007
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发表时间：
2012-10-01
影响因子：
1.7
通讯作者：
Balaban, Carey D.
中科院分区：
文献类型：
--
作者：
Ahn, Seong-Ki;Khalmuratova, Roza;Balaban, Carey D.
目的：本研究旨在通过免疫组织化学染色和逆转录聚合酶链反应检测大鼠前庭核中褪黑素1a（MT1a）和1b（MT1b）受体的定位和表达。材料和方法：本研究使用20只雄性Sprague-Dawley大鼠。 MTla和MT1b受体的抗体分别用于10只大鼠。另外10只动物被处死进行RT-PCR。从脑干切片中选择性分离含有内侧前庭核团的组织进行RT-PCR。结果：发现MTla和MT1b受体免疫阳性神经元分布在四个主要前庭核团中。两种受体主要在神经元体体及其近端树突中检测到。 RT-PCR 证实了内侧前庭核和三叉神经节中存在 MT1a 和 MT1b 受体 mRNA。结论：本研究首次证明 MTla 和 MT1b 受体在大鼠前庭核中定位并表达。这项研究提供了关于褪黑激素受体在前庭信号处理过程中的作用的更多见解。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Objective: The aim of this study was to examine the localizations and expressions of melatonin 1a (MT1a) and 1b (MT1b) receptors in rat vestibular nuclei by immunohistochemical staining and reverse transcriptase-polymerase chain reaction.Materials and methods: Twenty male Sprague-Dawley rats were used in this study. Antibodies for the MTla and MT1b receptors were used in 10 rats, respectively. A further 10 animals were sacrificed for RT-PCR. Tissues containing medial vestibular nuclei were selectively isolated from brain stem slices for RT-PCR.Results: MTla and MT1b receptor immunopositive neurons were found to be distributed throughout the four major vestibular nuclei. Both receptors were primarily detected in neuronal somata and their proximal dendrites. The presences of the mRNAs of the MT1a and MT1b receptors were confirmed by RT-PCR in medial vestibular nuclei and trigeminal ganglia.Conclusions: The present study demonstrates, for the first time, that MTla and MT1b receptors are localized and expressed in rat vestibular nuclei. This study provides additional insight into the role of melatonin receptors during vestibular signal processing. (C) 2011 Elsevier Ireland Ltd. All rights reserved.