Curcumin prevents the oxidation and lipid modification of LDL and its inhibition of prostacyclin generation by endothelial cells in culture
Curcumin prevents the oxidation and lipid modification of LDL and its inhibition of prostacyclin generation by endothelial cells in culture
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DOI：
10.1016/j.prostaglandins.2009.06.005
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发表时间：
2009-11-01
影响因子：
2.9
通讯作者：
Kummerow, Fred A.
中科院分区：
文献类型：
--
作者：
Mahfouz, Mohamedain M.;Zhou, Sherry Q.;Kummerow, Fred A.
从人血浆中分离出低密度脂蛋白 (LDL)，并在存在或不存在 1、3、5、10 或 20 μM 姜黄素的情况下，用 5 μM 硫酸铜在 37°C 下氧化 4 小时。在没有姜黄素的情况下氧化的 LDL (oxLDL) 显示出共轭二烯、脂质过氧化物 (TBARS) 和溶血卵磷脂 (lysoPC) 水平的增加以及多不饱和脂肪酸 (PUFA) 的显着损失。与oxLDL相比，在姜黄素存在下用5μM硫酸铜氧化LDL导致共轭二烯、脂质过氧化物、lysoPC显着减少以及PUFA显着增加。这些变化是剂量依赖性的，并且在 5 μM 姜黄素时达到最大值。将人内皮细胞(EC)与200μg蛋白/ml的oxLDL一起孵育导致前列环素(PGI(2))产生显着减少。与对照细胞相比，在 5 μM 姜黄素存在下氧化的 LDL 没有表现出对 PGI(2) 产生的任何抑制。这些结果表明姜黄素是一种有效的断链抗氧化剂，可防止 LDL 氧化和脂质修饰。 oxLDL对PGI(2)的抑制被认为是血栓形成和动脉粥样硬化发病机制中的一个促成因素。补充姜黄素可能是预防低密度脂蛋白氧化及其对动脉粥样硬化和病变形成的影响的有效策略。 (C) 2009 Elsevier Inc. 保留所有权利。
Low-density lipoprotein (LDL) was isolated from human plasma and oxidized by 5 mu M copper sulfate for 4 h at 37 degrees C in the absence and presence of 1, 3, 5, 10, or 20 mu M of curcumin. LDL oxidized in the absence of curcumin (oxLDL) showed an increased levels of conjugated dienes, lipid peroxides (TBARS) and lysolecithin (lysoPC) and a significant loss of polyunsaturated fatty acids (PUFA). LDL oxidized with 5 mu M copper sulfate in the presence of curcumin caused a significant decrease of conjugated diene, lipid peroxides, lysoPC and significant increase of PUFA compared to oxLDL. These changes were dose dependent and reached a maximum at 5 mu M curcumin. Incubation of human endothelial cells (EC) with 200 mu g protein/ml of oxLDL caused a significant decrease of prostacyclin (PGI(2)) generation. LDL oxidized in presence of 5 mu M curcumin did not show any inhibition of PGI(2) generation compared to the control cells. These results indicate that curcumin is an effective chain-breaking antioxidant which prevents oxidation and lipid modification of LDL. The inhibition of oxLDL on PGI(2) is considered a contributing factor in the pathogenesis of thrombosis and atherosclerosis. Curcumin supplementation could bean effective strategy in preventing LDL oxidation and its impact on atherosclerosis and lesion formation. (C) 2009 Elsevier Inc. All rights reserved.