Protective effects of epigallocatechin gallate after UV irradiation of cultured human lens epithelial cells.
Protective effects of epigallocatechin gallate after UV irradiation of cultured human lens epithelial cells.
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DOI：
10.3341/kjo.2008.22.3.183
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发表时间：
2008-09
影响因子：
--
通讯作者：
Koh JW
中科院分区：
文献类型：
--
作者：
Heo J;Lee BR;Koh JW
探讨没食子儿茶素没食子酸酯（EGCG）对体外培养的人晶状体上皮细胞紫外线照射的保护作用。我们用0.6 mW/cm2的紫外灯照射培养的人晶状体上皮细胞，脉冲时间为30秒。紫外线照射后5分钟和1小时，我们给药0、5、10、15、25、50或100 uM的EGCG。用显微镜计数室测定细胞数量，用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）测定细胞活力。与未处理的细胞相比，紫外线照射后培养的人晶状体上皮细胞总数明显增加。以剂量依赖的方式，egcg处理的细胞活力也更高。体外培养的人晶状体上皮细胞经紫外线照射后，EGCG增加了晶状体上皮细胞的数量和细胞活力，提示EGCG具有保护晶状体上皮免受紫外线损伤的作用。
To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells. We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells. EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.