Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay
Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay
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DOI：
10.1128/jcm.37.12.3860-3864.1999
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发表时间：
1999-12-01
影响因子：
9.4
通讯作者：
Stanley, J
中科院分区：
文献类型：
--
作者：
Lawson, AJ;Logan, JMJ;Stanley, J
基于 PCR 的人类肠道病原性弯曲杆菌感染发病率研究是基于检测和识别算法，包括筛选 PCR 和通过 PCR 酶联免疫吸附测定进行物种鉴定。该方法适用于从急性胃肠炎散发病例的 3,738 份粪便样本中提取的 DNA，这些样本由英格兰和威尔士的七个地区公共卫生实验室在两年内提交。发送实验室从 464 个样本中培养出了“弯曲杆菌属”。 PCR方法检测到492份弯曲杆菌阳性样本，培养和PCR相结合产生了543份弯曲杆菌阳性样本。 413 个样本存在同一性（重叠），但 79 个 PCR 阳性样本为培养阴性，51 个培养阳性样本为 PCR 阴性。虽然 PCR 和培养物在检测 C、空肠弯曲菌、大肠杆菌方面没有统计学上的显着差异（PCR，478 个样本；培养物，461 个样本），但 PCR 提供了有关混合感染和非 C、空肠弯曲菌和非 C 的独特数据。大肠杆菌弯曲杆菌。 19份样品中发现空肠弯曲菌和螺旋杆菌混合感染，1份样品中发现空肠弯曲菌和乌普萨拉弯曲菌混合感染；这在文化中并不明显。通过 PCR，11 例胃肠炎归因于乌普萨拉念珠菌，3 例归因于豚肠念珠菌，1 例归因于豚肠念珠菌，这代表了人类胃肠炎报道的豚肠念珠菌发病率最高，而拉里念珠菌的低发病率表明它在这方面不太重要。
A PCR-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening PCRs and species identification by PCR-enzyme-linked immunosorbent assay. This was applied to DNA extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven regional Public Health Laboratories in England and Wales over a 2-year period. The sending laboratories had cultured "Campylobacter spp," from 464 samples. The PCR methodologies detected 492 Campylobacter-positive samples, and the combination of culture and PCR yielded 543 Campylobacter-positive samples. There was identity (overlap) for 413 samples, but 79 PCR-positive samples were culture negative, and 51 culture-positive samples were PCR negative. While there was no statistically significant difference between PCR and culture in detection of C, jejuni-C, coli (PCR, 478 samples; culture, 461 samples), PCR provided unique data about mixed infections and non-C, jejuni and non-C. coli campylobacters. Mixed infections with C,jejuni and C, coil were found in 19 samples, and mixed infection with C,jejuni and C, upsaliensis was found in one sample; this was not apparent from culture. Eleven cases of gastroenteritis were attributed to C. upsaliensis by PCR, three cases were attributed to C, hyointestinalis, and one case was attributed to C, lari, This represents the highest incidence of C, hyointestinalis Set reported from human gastroenteritis, while the low incidence of C, lari suggests that it is less important in this context.