Enzyme-linked immunosorbent assay for detection of antibody to Leucocytozoon caulleryi.
Enzyme-linked immunosorbent assay for detection of antibody to Leucocytozoon caulleryi.
复制标题
用于检测白细胞虫考勒氏抗体的酶联免疫吸附测定。
DOI：
10.1080/03079458608436281
复制
发表时间：
1986
期刊：
影响因子：
2.8
通讯作者：
Kyo Suzuki
中科院分区：
文献类型：
--
作者：
Takashi Isobe;Kyo Suzuki
采用酶联免疫吸附试验(EL ISA)检测鸡血清中的卡氏白细胞体抗体。从抗原包被条件、最佳抗原浓度、血清和结合稀释度等方面对该方法进行了标准化。裂殖体抗原与微板聚苯乙烯孔表面结合或吸附有很强的亲和力。无特定病原的鸡血清和各种鸡病原抗血清均未观察到抗原的非特异性结合和交叉反应。酶联免疫吸附试验比间接免疫荧光抗体试验和琼脂沉淀试验更敏感。
An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Leucocytozoon caulleryi in chicken sera. The assay was standardised in terms of antigen coating conditions, optimum antigen concentration, serum and conjugate dilutions. The schizont antigen showed a strong affinity for binding or absorbing to the surface of the polystyrene wells of the microplate. Nonspecific binding of antigen and cross-reactions were not observed with sera from specific-pathogen-free chickens and antisera against various chicken disease agents. The ELISA was more sensitive than the indirect immuno-fluorescent antibody test and the agar gel precipitation test.