Effects of polymyxin B and Salmonella typhimurium antiserum on horses given endotoxin intravenously.
Effects of polymyxin B and Salmonella typhimurium antiserum on horses given endotoxin intravenously.
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多粘菌素 B 和鼠伤寒沙门氏菌抗血清对静脉注射内毒素的马的影响。
DOI：
10.2460/ajvr.1994.55.07.921
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发表时间：
1994
影响因子：
1
通讯作者：
Leslie A. Skelley
中科院分区：
文献类型：
--
作者：
Mary M. Durando;R. MacKay;Stephen Linda;Leslie A. Skelley
在马内毒素血症模型中评估了多粘菌素 B 和针对 Re 突变型鼠伤寒沙门氏菌的抗血清的保护作用。六匹 3 至 5 月龄的小马驹在未预处理或用多粘菌素 B（6,000 U/kg，IV）或鼠伤寒沙门氏菌抗血清（1.5 ml/kg，IV）预处理后静脉注射内毒素（0.25 微克/千克体重）。如果未经预处理，内毒素会导致短暂的躺卧并导致直肠温度、心率和呼吸频率升高。此外，还检测到白细胞减少以及循环肿瘤坏死因子 (TNF) 和白细胞介素 6 (IL-6) 活性的增加。与单独给予内毒素时获得的结果相比，多粘菌素B预处理导致最大血浆TNF和IL-6活性显着降低（P < 0.05），并且直肠温度和呼吸频率显着降低。相反，与未经预处理的内毒素作用相比，使用抗血清与显着（P < 0.05）较高的呼吸频率、最大血浆IL-6活性和总TNF反应（根据血浆TNF与时间的曲线下面积确定）相关。这些结果表明多粘菌素 B 可能具有治疗马内毒素血症的潜力。鼠伤寒沙门氏菌抗血清在此模型中没有积极作用，并且在某些条件下可能会加剧内毒素的作用。
Polymyxin B and an antiserum against an Re mutant Salmonella typhimurium were evaluated for protective effect in an equine model endotoxemia. Six 3- to 5-month-old foals were given endotoxin (0.25 micrograms/kg of body weight) IV after no pretreatment, or pretreatment with polymyxin B (6,000 U/kg, IV) or S typhimurium antiserum (1.5 ml/kg, IV). When given without pretreatment, endotoxin caused transient recumbency and increases in rectal temperature, and heart and respiratory rates. In addition, leukopenia and increases in circulating tumor necrosis factor (TNF) and interleukin 6 (IL-6) activities were detected. Compared with results obtained when endotoxin was given alone, pretreatment with polymyxin B resulted in significantly (P < 0.05) lower maximal plasma TNF and IL-6 activities, and significantly lower rectal temperature and respiratory rate. In contrast, compared with effects of endotoxin given without pretreatment, use of antiserum was associated with significantly (P < 0.05) higher respiratory rate, maximal plasma IL-6 activity, and total TNF response (as determined by areas under curves of plasma TNF vs time). These results indicate that polymyxin B may have potential as a treatment for equine endotoxemia. Salmonella typhimurium antiserum had no positive effect in this model, and, under certain conditions, may exacerbate the actions of endotoxin.