Lipid peroxidation by human blood phagocytes.
Lipid peroxidation by human blood phagocytes.
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人血液吞噬细胞的脂质过氧化作用。
DOI：
10.1172/jci107801
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发表时间：
1974
影响因子：
15.9
通讯作者：
A. L. Smith
中科院分区：
文献类型：
--
作者：
T. Stossel;R. Mason;A. L. Smith
从正常外周血中通过沉淀在泛影葡钠-菲科尔梯度上或从中性粒细胞减少和单核细胞增多症患者的血液中获得的富含人血单核细胞的细胞悬浮液，在与聚苯乙烯珠子或热致死的表皮葡萄球菌孵育期间积累丙二醛，丙二醛是一种不稳定的脂质过氧化分解代谢产物。混合血白细胞主要由粒细胞或经密度梯度沉淀法纯化的粒细胞组成，在与这些颗粒孵育过程中不积累丙二醛，但在摄入含有亚麻酸酯的颗粒时积累丙二醛。比较了来自相同捐献者的单核细胞浓缩和纯化的粒细胞制剂的磷脂脂肪酸组成。富含单核细胞的磷脂中花生四烯酸的摩尔分数(20：4)比纯化的粒细胞高62%。研究结果表明，人类单核细胞在内吞过程中，可能由于其细胞膜中含有更多的多不饱和脂肪酸，比粒细胞更容易氧化更多的内源性脂肪。正常人的粒细胞具有氧化摄入的脂肪的能力。然而，来自两名慢性肉芽肿性疾病患者的混合白细胞在吞噬含有亚麻酸的颗粒时产生的丙二醛很少。因此，脂质过氧化的能力与细胞氧代谢有关，这是慢性肉芽肿性疾病粒细胞不足的一种功能。由四甲氧基丙烷水解制得的丙二醛、从过氧化亚麻酸中提取的丙二醛或从吞噬兔肺泡巨噬细胞提取物中提纯的丙二醛对大肠杆菌和表皮葡萄球菌具有杀菌活性。因此，脂质过氧化氢的毒性分解代谢产物可能会增强过氧化氢对单核吞噬细胞的杀菌活性。
Cell suspensions enriched in human blood monocytes, obtained from normal peripheral blood by sedimentation on sodium diatrizoate-Ficoll gradients or from the blood of patients with neutropenia and monocytosis, accumulated malonyldialdehyde, a labile catabolite of lipid peroxidation, during incubations with polystyrene beads or heat-killed Staphylococcus epidermidis. Mixed blood leukocytes principally composed of granulocytes or granulocytes purified by density gradient sedimentation did not accumulate malonyldialdehyde during incubations with these particles, but did when ingesting particles containing linolenate. The phospholipid fatty acid composition of monocyte-enriched and purified granulocyte preparations from the same donors were compared. The molar fraction of arachidonate (20:4) in phospholipids from monocyte-rich preparations was 62% greater than that of purified granulocytes. The findings indicate that human monocytes, possibly because of a greater content of polyunsaturated fatty acids in their membranes, peroxidize a greater quantity of endogenous lipids than granulocytes during endocytosis. Normal human granulocytes have the capacity to peroxidize ingested lipids. However, mixed leukocytes from two patients with chronic granulomatous disease produced little malonyldialdehyde when engulfing linolenate-containing particles. Therefore the capacity to peroxidize lipid is related to cellular oxygen metabolism, a function in which chronic granulomatous disease granulocytes are dificient. Malonyldialdehyde chemically prepared by hydrolysis of tetramethoxypropane, by extraction from peroxidized linolenic acid, or purified from extracts of phagocytizing rabbit alveolar macrophages had bactericidal activity against Escherichia coli and S. epidermidis. Therefore, toxic catabolites of lipid hydroperoxides may potentiate the bactericidal activity of hydrogen peroxide in mononuclear phagocytes.