ZmHO-1, a maize haem oxygenase-1 gene, plays a role in determining lateral root development
ZmHO-1, a maize haem oxygenase-1 gene, plays a role in determining lateral root development
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DOI：
10.1016/j.plantsci.2011.12.012
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发表时间：
2012-03-01
期刊：
影响因子：
5.2
通讯作者：
Xie, Gui-Shui
中科院分区：
文献类型：
--
作者：
Han, Bin;Xu, Sheng;Xie, Gui-Shui
已有研究表明，HO-1/CO系统参与了生长素诱导不定根的形成。本研究从玉米幼苗中克隆了编码HO-1蛋白的基因ZmHO-1。ZmHO-1具有保守的HO特征序列，与水稻SE5(Osho-1)蛋白具有最高的同源性。我们进一步发现，N-1-萘乙酸(NAA)、胡敏和CO水溶液诱导了ZmHO-1的表达，从而促进了侧根的发育。这些作用是ZmHO-1特有的，因为有效的HO-1抑制剂锌原卟啉IX(ZnPPIX)以不同的方式阻断了上述作用。添加胡敏素和CO能够逆转生长素耗竭引起的侧根形成抑制以及ZmHO-1转录本的减少。分子证据表明，参与侧根形成的目的基因如ZmCDK和ZmCK12的调控可以被ZnPPIX阻断。ZmHO-1在转基因拟南芥中的过表达促进了侧根的发育，并调控了细胞周期调控基因的表达。总体而言，我们的结果表明玉米HO-1基因是侧根形成所必需的。(C)2011爱思唯尔爱尔兰有限公司。保留所有权利。
Previous results revealed that haem oxygenase-1 (HO-1)/carbon monoxide (CO) system is involved in auxin-induced adventitious root formation. In this report, a cDNA for the gene ZmHO-1, encoding an HO-1 protein, was cloned from Zea mays seedlings. ZmHO-1 has a conserved HO signature sequence and shares highest homology with rice SE5 (OsHO-1) protein. We further discovered that N-1-naphthylacetic acid (NAA), haemin, and CO aqueous solution, led to the induction of ZmHO-1 expression as well as the thereafter promotion of lateral root development. These effects were specific for ZmHO-1 since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) differentially blocked the above actions. The addition of haemin and CO were able to reverse the auxin depletion-triggered inhibition of lateral root formation as well as the decreased ZmHO-1 transcripts. Molecular evidence showed that the haemin- or CO-mediated the modulation of target genes responsible for lateral root formation, including ZmCDK and ZmCK12, could be blocked by ZnPPIX. Overexpression of ZmHO-1 in transgenic Arabidopsis plants resulted in promotion of lateral root development as well as the modulation of cell cycle regulatory gene expressions. Overall, our results suggested that a maize HO-1 gene is required for the lateral root formation. (C) 2011 Elsevier Ireland Ltd. All rights reserved.