Co-cultured Production of Lignin-Modifying Enzymes with White-Rot Fungi
Co-cultured Production of Lignin-Modifying Enzymes with White-Rot Fungi
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DOI：
10.1007/s12010-011-9289-9
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Zibek, Susanne
中科院分区：
文献类型：
--
作者：
Chen Qi-he;Kruegener, Sven;Zibek, Susanne
共培养是一种潜在的木质纤维素降解策略，由于它们之间的协同作用，可以产生高活性的酶。本研究的目的是调查很少了解的两种白腐真菌的共培养对木质素修饰酶（LME）的生产的影响。本研究选取了六个物种，Bjerkandera adusta、Phlebia radiata、Pleoprostreatus、Dichomitus squalens、Hypoxylon fragiforme和Pleoproerynalus，成对培养以研究LME的产生。观察到的成对菌丝的相互作用表明，P. erynatum不适合共生长。使用含有染料RBBR的琼脂平板显示在菌丝体之间的对峙区的脱色提高。漆酶只有在辐射藻与D.在深层培养下的角鲨烷;最高值在孵育4天后测得（120 U mg（-1））。在糙皮侧耳和辐射侧耳共培养时（培养4 d后MnP = 800 nkat L-1;培养7 d后LiP = 60 nkat L-1），同时观察到MnP和LiP产量的提高，但其漆酶产量并不高。糙皮侧耳似乎具有用于共培养生产大型海洋生态系统的特定潜力。LME的表型不仅取决于所使用的物种，而且还受培养基中不同营养物质的调节。本研究结果为阐明C/N在共培养条件下对LME生产的调控作用提供了证据。
Co-cultivation was a potential strategy in lignocellulolytic biodegradation with producing high activity enzymes due to their synergistic action. The objective of this study was to investigate the rarely understood effects of co-culturing of two white-rot fungi on lignin-modifying enzymes (LMEs) production. Six species, Bjerkandera adusta, Phlebia radiata, Pleurotus ostreatus, Dichomitus squalens, Hypoxylon fragiforme and Pleurotus eryngii, were cultured in pairs to study the production of LMEs. The paired hyphal interaction observed showed that P. eryngii is not suitable for co-growth. The use of agar plates containing dye RBBR showed elevated decolourisation at the confrontation zone between mycelia. Laccase was significantly stimulated only in the co-culture of P. radiata with D. squalens under submerged cultivation; the highest value was measured after 4 days of incubation (120 U mg(-1)). The improved productions of MnP and LiP were simultaneously observed at the co-culture of P. ostreatus and P. radiata (MnP = 800 nkat L-1 after 4 days of incubation; LiP = 60 nkat L-1 after 7 days of incubation), though it was not a good producer of laccase. P. ostreatus appeared to possess specific potential to be used in co-cultured production of LMEs. The phenotype of LMEs production was not only dependent on the species used but also regulated by different nutritions available in the culture medium. The present data will provide evidence for illustrating the regulatory roles of C/N on LMEs production under the co-cultures' circumstances.