Expression of heat shock protein 90 genes during early development and infection in Megalobrama amblycephala and evidence for adaptive evolution in teleost
Expression of heat shock protein 90 genes during early development and infection in Megalobrama amblycephala and evidence for adaptive evolution in teleost
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DOI：
10.1016/j.dci.2013.08.007
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发表时间：
2013-12-01
影响因子：
2.9
通讯作者：
Liu, Hong
中科院分区：
文献类型：
--
作者：
Ding, Zhujin;Wu, Junjie;Liu, Hong
热休克蛋白90（HSP 90）是一种高度保守的分子伴侣，在细胞发育过程中起着关键作用。在这项研究中，我们报告了Hsp 90 α和Hsp 90 β基因在团头鲂，在早期发育过程中的表达谱，在各种健康组织和响应细菌感染，和评估其适应性进化的特征。Hsp 90 α cDNA的开放阅读框为2193 bp，编码731个氨基酸，Hsp 90 β cDNA的开放阅读框为2184 bp，编码728个氨基酸。实时荧光定量PCR（qRT-PCR）分析表明，Hsp 90 α和Hsp 90 β的mRNA在孵化后15 d达到最高水平。使用qRT-PCR和Western blot，Hsp 90 α和Hsp 90 β在各种健康组织中广泛表达，并且在血液中显著高于其他组织。Hsp 90 α和Hsp 90 β的表达在细菌感染后均上调，并在感染后4 h达到峰值水平。位点模型分析表明，在Hsp 90 α中存在一个正选择位点（T717），而在Hsp 90 β中没有发现正选择位点。分支-位点模型检验表明，Hsp 90 α基因在鲑形目和腹鱼形目分支中存在适应性进化证据，Hsp 90 β基因在鲑形目分支中存在适应性进化证据。(C)2013爱思唯尔有限公司保留所有权利。
Heat shock protein 90 (HSP90) are highly conserved molecular chaperones, playing a pivotal role in cellular progress. In this study, we reported the characterization of the Hsp90 alpha and Hsp90 beta genes in Megalobrama amblycephala, the expression profiling during early development, in various healthy tissues and in response to bacterial infection, and the assessment of their adaptive evolution. The Hsp90 alpha cDNA contains an open reading frame (ORF) of 2193 bp encoding 731 amino acids and the Hsp90 beta cDNA has an ORF of 2184 bp encoding 728 amino acids. Using quantitative real-time PCR (qRT-PCR) analysis, the mRNA of both Hsp90a and Hsp90 beta reached the highest level at 15th day post-hatch. Using qRT-PCR and Western blot, both Hsp90 alpha and Hsp90 beta were widely expressed in various healthy tissues and significantly higher in blood than in other tissues. Expression of both Hsp90 alpha and Hsp90 beta were up-regulated upon bacterial infection and reached the peak level at 4 h post infection. Site model analysis indicated that one positive selection site (T717) in Hsp90 alpha was found, while no positive selection site was observed in Hsp90 beta. Branch-site model test showed that there were adaptively evolutionary evidences in the branches of Salmoniformes and Gasterosteiformes for Hsp90 alpha gene, and in the branch of Salmoniformes for Hsp90 beta gene. (C) 2013 Elsevier Ltd. All rights reserved.